Gene description for Ppm1b
Gene name protein phosphatase, Mg2+/Mn2+ dependent, 1B
Gene symbol Ppm1b
Other names/aliases Pp2c2
Species Rattus norvegicus
 Database cross references - Ppm1b
ExoCarta ExoCarta_24667
Entrez Gene 24667
UniProt P35815  
 Ppm1b identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Ppm1b
Molecular Function
    protein serine/threonine phosphatase activity GO:0004722 ISO
    manganese ion binding GO:0030145 IEA
    magnesium ion binding GO:0000287 IEA
Biological Process
    N-terminal protein myristoylation GO:0006499 ISS
    peptidyl-threonine dephosphorylation GO:0035970 ISO
    negative regulation of NF-kappaB import into nucleus GO:0042347 ISS
    protein dephosphorylation GO:0006470 ISS
    negative regulation of defense response to virus GO:0050687 ISS
    negative regulation of interferon-beta production GO:0032688 ISS
    negative regulation of I-kappaB kinase/NF-kappaB signaling GO:0043124 ISS
Subcellular Localization
    cytosol GO:0005829 ISS
    membrane GO:0016020 ISS
 Experiment description of studies that identified Ppm1b in exosomes
1
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ppm1b
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ppm1b is involved
PathwayEvidenceSource
ISG15 antiviral mechanism IEA Reactome





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