Gene description for Vps4a
Gene name vacuolar protein sorting 4 homolog A (S. cerevisiae)
Gene symbol Vps4a
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Vps4a
ExoCarta ExoCarta_246772
Entrez Gene 246772
UniProt Q793F9  
 Vps4a identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Vps4a
Molecular Function
    protein C-terminus binding GO:0008022 ISO
    Rho GTPase binding GO:0017048 IPI
    microtubule-severing ATPase activity GO:0008568 IBA
    ATP binding GO:0005524 ISO
    protein domain specific binding GO:0019904 ISO
    ATPase activity GO:0016887 ISO
Biological Process
    viral release from host cell GO:0019076 ISO
    vacuole organization GO:0007033 IBA
    positive regulation of viral process GO:0048524 ISO
    ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043162 ISO
    ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway GO:0090611 ISO
    vesicle uncoating GO:0072319 ISO
    positive regulation of viral budding via host ESCRT complex GO:1903774 ISO
    cell separation after cytokinesis GO:0000920 ISO
    positive regulation of exosomal secretion GO:1903543 ISO
    intracellular cholesterol transport GO:0032367 ISO
    membrane budding GO:0006900 ISO
    nucleus organization GO:0006997 ISO
    viral budding via host ESCRT complex GO:0039702 ISO
    abscission GO:0009838 ISS
    vesicle-mediated transport GO:0016192 ISO
    protein transport GO:0015031 IEA
    positive regulation of viral life cycle GO:1903902 ISO
    endosomal transport GO:0016197 ISO
    positive regulation of viral release from host cell GO:1902188 ISO
    mitotic metaphase plate congression GO:0007080 ISO
    cytoplasmic microtubule organization GO:0031122 IBA
    endosomal vesicle fusion GO:0034058 ISO
    negative regulation of cytokinesis GO:0032466 ISS
    metabolic process GO:0008152 IEA
    regulation of protein localization GO:0032880 ISO
    cell division GO:0051301 ISO
    regulation of protein localization to plasma membrane GO:1903076 ISO
Subcellular Localization
    early endosome GO:0005769 ISO
    ESCRT III complex GO:0000815 ISO
    late endosome GO:0005770 ISO
    late endosome membrane GO:0031902 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
    nucleus GO:0005634 ISO
    spindle pole GO:0000922 ISO
    Flemming body GO:0090543 ISO
    extracellular exosome GO:0070062 ISO
    centrosome GO:0005813 ISO
    cytosol GO:0005829 ISO
    vacuolar membrane GO:0005774 ISO
    lysosome GO:0005764 ISO
    plasma membrane GO:0005886 ISO
    endosome GO:0005768 ISO
    midbody GO:0030496 ISS
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Vps4a in exosomes
1
Experiment ID 90
ISEV standards
EM
EV Biophysical techniques
HSC70|HSP90|TSG101
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Vps4a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Vps4a is involved
PathwayEvidenceSource
Endosomal Sorting Complex Required For Transport (ESCRT) IEA Reactome





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