Gene description for Prkar2b
Gene name protein kinase, cAMP dependent regulatory, type II beta
Gene symbol Prkar2b
Other names/aliases RATDNA
Species Rattus norvegicus
 Database cross references - Prkar2b
ExoCarta ExoCarta_24679
Entrez Gene 24679
UniProt P12369  
 Prkar2b identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
 Gene ontology annotations for Prkar2b
Molecular Function
    protein binding GO:0005515 IPI
    ubiquitin protein ligase binding GO:0031625 ISO
    cAMP-dependent protein kinase regulator activity GO:0008603 ISO
    protein kinase A catalytic subunit binding GO:0034236 ISO
    cAMP binding GO:0030552 IDA
    cAMP-dependent protein kinase inhibitor activity GO:0004862 ISO
    protein domain specific binding GO:0019904 IMP
    protein kinase binding GO:0019901 ISO
Biological Process
    response to antipsychotic drug GO:0097332 IEP
    regulation of protein kinase activity GO:0045859 ISO
    learning GO:0007612 ISO
    fatty acid metabolic process GO:0006631 ISO
    negative regulation of cAMP-dependent protein kinase activity GO:2000480 ISO
    response to clozapine GO:0097338 IEP
Subcellular Localization
    perinuclear region of cytoplasm GO:0048471 IDA
    ciliary base GO:0097546 ISO
    centrosome GO:0005813 ISO
    cytoplasm GO:0005737 ISO
    cAMP-dependent protein kinase complex GO:0005952 IEA
    dendritic shaft GO:0043198 ISS
    dendrite GO:0030425 IDA
    plasma membrane GO:0005886 ISO
    membrane raft GO:0045121 IDA
    neuronal cell body GO:0043025 IDA
    extracellular exosome GO:0070062 ISO
    mitochondrial inner membrane GO:0005743 IDA
    dendritic spine GO:0043197 ISS
 Experiment description of studies that identified Prkar2b in exosomes
1
Experiment ID 225
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Prkar2b
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Prkar2b is involved
PathwayEvidenceSource
Glucagon-like Peptide-1 (GLP1) regulates insulin secretion IEA Reactome





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