Gene description for Prkar2b
Gene name protein kinase, cAMP dependent regulatory, type II beta
Gene symbol Prkar2b
Other names/aliases RATDNA
Species Rattus norvegicus
 Database cross references - Prkar2b
ExoCarta ExoCarta_24679
Vesiclepedia VP_24679
Entrez Gene 24679
UniProt P12369  
 Prkar2b identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
 Gene ontology annotations for Prkar2b
Molecular Function
    cAMP-dependent protein kinase inhibitor activity GO:0004862 IBA
    cAMP-dependent protein kinase inhibitor activity GO:0004862 ISO
    protein binding GO:0005515 IPI
    cAMP-dependent protein kinase regulator activity GO:0008603 IDA
    cAMP-dependent protein kinase regulator activity GO:0008603 IEA
    cAMP-dependent protein kinase regulator activity GO:0008603 ISO
    kinase activity GO:0016301 IEA
    protein kinase binding GO:0019901 ISO
    protein domain specific binding GO:0019904 IDA
    cAMP binding GO:0030552 IBA
    cAMP binding GO:0030552 IDA
    cAMP binding GO:0030552 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    protein kinase A catalytic subunit binding GO:0034236 IBA
    protein kinase A catalytic subunit binding GO:0034236 IC
    protein kinase A catalytic subunit binding GO:0034236 ISO
Biological Process
    fatty acid metabolic process GO:0006631 ISO
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IBA
    learning GO:0007612 ISO
    regulation of protein kinase activity GO:0045859 IDA
    modulation of chemical synaptic transmission GO:0050804 ISO
    response to antipsychotic drug GO:0097332 IEP
    negative regulation of cAMP/PKA signal transduction GO:0141162 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    centrosome GO:0005813 ISO
    cytosol GO:0005829 IBA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    cAMP-dependent protein kinase complex GO:0005952 IBA
    cAMP-dependent protein kinase complex GO:0005952 IDA
    cAMP-dependent protein kinase complex GO:0005952 IEA
    cAMP-dependent protein kinase complex GO:0005952 ISO
    dendrite GO:0030425 IDA
    neuronal cell body GO:0043025 IDA
    dendritic spine GO:0043197 ISO
    dendritic spine GO:0043197 ISS
    dendritic shaft GO:0043198 ISO
    dendritic shaft GO:0043198 ISS
    perinuclear region of cytoplasm GO:0048471 IDA
    ciliary base GO:0097546 ISO
    postsynapse GO:0098794 ISO
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Prkar2b in sEVs
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Prkar2b
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Prkar2b is involved
PathwayEvidenceSource
Activation of NMDA receptors and postsynaptic events IEA Reactome
Anchoring of the basal body to the plasma membrane IEA Reactome
Aquaporin-mediated transport IEA Reactome
AURKA Activation by TPX2 IEA Reactome
Ca-dependent events IEA Reactome
Calmodulin induced events IEA Reactome
CaM pathway IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular responses to mechanical stimuli IEA Reactome
Cellular responses to stimuli IEA Reactome
Centrosome maturation IEA Reactome
Cilium Assembly IEA Reactome
CREB1 phosphorylation through the activation of Adenylate Cyclase IEA Reactome
DAG and IP3 signaling IEA Reactome
DARPP-32 events IEA Reactome
Factors involved in megakaryocyte development and platelet production IEA Reactome
G alpha (i) signalling events IEA Reactome
G alpha (s) signalling events IEA Reactome
G-protein mediated events IEA Reactome
G2/M Transition IEA Reactome
Glucagon signaling in metabolic regulation IEA Reactome
GPCR downstream signalling IEA Reactome
GPER1 signaling IEA Reactome
Hedgehog 'off' state IEA Reactome
Hemostasis IEA Reactome
High laminar flow shear stress activates signaling by PIEZO1 and PECAM1:CDH5:KDR in endothelial cells IEA Reactome
Integration of energy metabolism IEA Reactome
Intracellular signaling by second messengers IEA Reactome
Loss of Nlp from mitotic centrosomes IEA Reactome
Loss of proteins required for interphase microtubule organization from the centrosome IEA Reactome
M Phase IEA Reactome
Metabolism IEA Reactome
Mitotic G2-G2/M phases IEA Reactome
Mitotic Prometaphase IEA Reactome
Neuronal System IEA Reactome
Neurotransmitter receptors and postsynaptic signal transmission IEA Reactome
Opioid Signalling IEA Reactome
Organelle biogenesis and maintenance IEA Reactome
PKA activation IEA Reactome
PKA activation in glucagon signalling IEA Reactome
PKA-mediated phosphorylation of CREB IEA Reactome
PLC beta mediated events IEA Reactome
Post NMDA receptor activation events IEA Reactome
Recruitment of mitotic centrosome proteins and complexes IEA Reactome
Recruitment of NuMA to mitotic centrosomes IEA Reactome
Regulation of PLK1 Activity at G2/M Transition IEA Reactome
Response of endothelial cells to shear stress IEA Reactome
Signal Transduction IEA Reactome
Signaling by GPCR IEA Reactome
Signaling by Hedgehog IEA Reactome
Transmission across Chemical Synapses IEA Reactome
Transport of small molecules IEA Reactome
Vasopressin regulates renal water homeostasis via Aquaporins IEA Reactome





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