Gene description for Prps2
Gene name phosphoribosyl pyrophosphate synthetase 2
Gene symbol Prps2
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Prps2
ExoCarta ExoCarta_24689
Vesiclepedia VP_24689
Entrez Gene 24689
UniProt P09330  
 Prps2 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Prps2
Molecular Function
    magnesium ion binding GO:0000287 IDA
    magnesium ion binding GO:0000287 IEA
    ribose phosphate diphosphokinase activity GO:0004749 IBA
    ribose phosphate diphosphokinase activity GO:0004749 IDA
    ribose phosphate diphosphokinase activity GO:0004749 IEA
    ribose phosphate diphosphokinase activity GO:0004749 ISO
    ribose phosphate diphosphokinase activity GO:0004749 ISS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISS
    AMP binding GO:0016208 IDA
    kinase activity GO:0016301 IEA
    GDP binding GO:0019003 IDA
    carbohydrate binding GO:0030246 IDA
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 ISS
    ADP binding GO:0043531 IDA
Biological Process
    5-phosphoribose 1-diphosphate biosynthetic process GO:0006015 IBA
    5-phosphoribose 1-diphosphate biosynthetic process GO:0006015 IDA
    5-phosphoribose 1-diphosphate biosynthetic process GO:0006015 IEA
    5-phosphoribose 1-diphosphate biosynthetic process GO:0006015 ISO
    pentose-phosphate shunt GO:0006098 IEA
    pentose-phosphate shunt GO:0006098 ISO
    purine nucleotide biosynthetic process GO:0006164 IBA
    AMP biosynthetic process GO:0006167 IDA
    ribonucleoside monophosphate biosynthetic process GO:0009156 IEA
    nucleotide biosynthetic process GO:0009165 IEA
    animal organ regeneration GO:0031100 IEP
Subcellular Localization
    ribose phosphate diphosphokinase complex GO:0002189 IDA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    protein-containing complex GO:0032991 IDA
 Experiment description of studies that identified Prps2 in sEVs
1
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Prps2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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