ExoCarta: Gene summary

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Gene description for RT1-M3-1
Gene name	RT1 class Ib, locus M3, gene 1
Gene symbol	RT1-M3-1
Other names/aliases	4B2/3.7 H2-M3 RT1-M3 RanoM3 RanoM301 RanoM302
Species	Rattus norvegicus
Database cross references - RT1-M3-1
ExoCarta	ExoCarta_24747
Entrez Gene	24747
RT1-M3-1 identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for RT1-M3-1

Molecular Function
	peptide antigen binding	GO:0042605	IBA
	receptor binding	GO:0005102	IBA
Biological Process
	alpha-beta T cell activation involved in immune response	GO:0002287	IEA
	antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent	GO:0002481	IEA
	positive regulation of T cell mediated cytotoxicity	GO:0001916	IEA
	response to molecule of bacterial origin	GO:0002237	IEA
	positive regulation of natural killer cell mediated cytotoxicity	GO:0045954	IEA
	antigen processing and presentation of endogenous peptide antigen via MHC class Ib	GO:0002476	IEA
	defense response to bacterium	GO:0042742	IEA
	antigen processing and presentation of peptide antigen via MHC class I	GO:0002474	IBA
	positive regulation of nitric oxide biosynthetic process	GO:0045429	IEA
	positive regulation of interferon-gamma production	GO:0032729	IEA
Subcellular Localization
	external side of plasma membrane	GO:0009897	IEA
	MHC class Ib protein complex	GO:0032398	IEA
	MHC class I protein complex	GO:0042612	IEA
	plasma membrane	GO:0005886	IBA
	integral component of membrane	GO:0016021	IEA

Experiment description of studies that identified RT1-M3-1 in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ HSC70\|HSP90\|TSG101	EV Cytosolic markers
✔ MHCI	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Western blotting

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D2 Rep 1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.16-1.21 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting