Gene description for Slc2a1
Gene name solute carrier family 2 (facilitated glucose transporter), member 1
Gene symbol Slc2a1
Other names/aliases GLUTB
GTG1
Glut1
Gtg3
RATGTG1
Species Rattus norvegicus
 Database cross references - Slc2a1
ExoCarta ExoCarta_24778
Vesiclepedia VP_24778
Entrez Gene 24778
UniProt P11167  
 Slc2a1 identified in sEVs derived from the following tissue/cell type
Hepatocytes 19367702    
Reticulocytes 21828046    
 Gene ontology annotations for Slc2a1
Molecular Function
    long-chain fatty acid transmembrane transporter activity GO:0005324 ISO
    protein binding GO:0005515 IPI
    fucose transmembrane transporter activity GO:0015150 ISO
    kinase binding GO:0019900 IPI
    dehydroascorbic acid transmembrane transporter activity GO:0033300 IDA
    dehydroascorbic acid transmembrane transporter activity GO:0033300 ISO
    identical protein binding GO:0042802 ISO
    xenobiotic transmembrane transporter activity GO:0042910 IDA
    D-glucose transmembrane transporter activity GO:0055056 IBA
    D-glucose transmembrane transporter activity GO:0055056 IDA
    D-glucose transmembrane transporter activity GO:0055056 IEA
    D-glucose transmembrane transporter activity GO:0055056 ISO
Biological Process
    response to hypoxia GO:0001666 IEP
    central nervous system development GO:0007417 ISO
    female pregnancy GO:0007565 IEP
    long-chain fatty acid import across plasma membrane GO:0015911 ISO
    cerebral cortex development GO:0021987 IEP
    response to insulin GO:0032868 IBA
    response to insulin GO:0032868 IEP
    cellular response to glucose starvation GO:0042149 ISO
    xenobiotic transport GO:0042908 IDA
    photoreceptor cell maintenance GO:0045494 ISO
    photoreceptor cell maintenance GO:0045494 ISS
    D-glucose import GO:0046323 IBA
    D-glucose import GO:0046323 IMP
    protein-containing complex assembly GO:0065003 ISO
    protein-containing complex assembly GO:0065003 ISS
    dehydroascorbic acid transport GO:0070837 IBA
    dehydroascorbic acid transport GO:0070837 IDA
    dehydroascorbic acid transport GO:0070837 ISO
    cellular response to mechanical stimulus GO:0071260 IEP
    cellular hyperosmotic response GO:0071474 IEP
    D-glucose import across plasma membrane GO:0098708 ISO
    transport across blood-brain barrier GO:0150104 ISO
    transport across blood-brain barrier GO:0150104 ISO
    response to Thyroglobulin triiodothyronine GO:1904016 IEP
    D-glucose transmembrane transport GO:1904659 IDA
    D-glucose transmembrane transport GO:1904659 IEA
    D-glucose transmembrane transport GO:1904659 ISO
Subcellular Localization
    Golgi membrane GO:0000139 ISO
    female germ cell nucleus GO:0001674 ISO
    photoreceptor inner segment GO:0001917 IEA
    photoreceptor inner segment GO:0001917 ISO
    female pronucleus GO:0001939 ISO
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    caveola GO:0005901 IDA
    cell-cell junction GO:0005911 IDA
    intercalated disc GO:0014704 IDA
    membrane GO:0016020 ISO
    basolateral plasma membrane GO:0016323 IBA
    basolateral plasma membrane GO:0016323 IDA
    basolateral plasma membrane GO:0016323 ISO
    apical plasma membrane GO:0016324 IBA
    apical plasma membrane GO:0016324 IDA
    apical plasma membrane GO:0016324 ISO
    Z disc GO:0030018 IDA
    midbody GO:0030496 ISO
    cortical actin cytoskeleton GO:0030864 ISO
    cortical actin cytoskeleton GO:0030864 ISS
    vesicle GO:0031982 ISO
    sarcolemma GO:0042383 IDA
    membrane raft GO:0045121 ISO
    synapse GO:0045202 ISO
    presynapse GO:0098793 ISO
    glucose transporter complex GO:1990350 ISO
 Experiment description of studies that identified Slc2a1 in sEVs
1
Experiment ID 35
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|CD81|CD63
Enriched markers
HSPA5
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Slc2a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
2 Ubc  
Affinity Capture-Western Rattus norvegicus
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