Gene description for Sparc
Gene name secreted protein, acidic, cysteine-rich (osteonectin)
Gene symbol Sparc
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Sparc
ExoCarta ExoCarta_24791
Entrez Gene 24791
UniProt P16975  
 Sparc identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
 Gene ontology annotations for Sparc
Molecular Function
    collagen binding GO:0005518 ISO
    calcium ion binding GO:0005509 ISO
    extracellular matrix binding GO:0050840 ISO
Biological Process
    response to cytokine GO:0034097 IEP
    signal transduction GO:0007165 IEA
    response to lead ion GO:0010288 IEP
    negative regulation of endothelial cell proliferation GO:0001937 ISO
    lung development GO:0030324 IMP
    cellular response to growth factor stimulus GO:0071363 ISO
    response to calcium ion GO:0051592 IEP
    positive regulation of endothelial cell migration GO:0010595 ISO
    negative regulation of angiogenesis GO:0016525 ISO
    heart development GO:0007507 IEP
    response to gravity GO:0009629 IEP
    regulation of cell proliferation GO:0042127 ISO
    wound healing GO:0042060 IEP
    regulation of cell morphogenesis GO:0022604 ISO
    response to peptide hormone GO:0043434 IEP
    ossification GO:0001503 IEP
    response to glucocorticoid GO:0051384 IEP
    bone development GO:0060348 ISO
    response to lipopolysaccharide GO:0032496 IEP
    response to L-ascorbic acid GO:0033591 IEP
    response to cAMP GO:0051591 IEP
    response to cadmium ion GO:0046686 IEP
    response to ethanol GO:0045471 IEP
    inner ear development GO:0048839 IEP
Subcellular Localization
    basement membrane GO:0005604 IEA
    mitochondrion GO:0005739 ISO
    intracellular GO:0005622 IDA
    membrane-bounded vesicle GO:0031988 IDA
    proteinaceous extracellular matrix GO:0005578 ISO
    nucleus GO:0005634 IDA
    plasma membrane GO:0005886 ISO
    cytoplasm GO:0005737 ISO
    cell surface GO:0009986 ISO
    platelet alpha granule GO:0031091 ISO
    nuclear matrix GO:0016363 ISO
    platelet alpha granule membrane GO:0031092 ISO
    extracellular space GO:0005615 IDA
 Experiment description of studies that identified Sparc in exosomes
1
Experiment ID 225
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for Sparc
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Sparc is involved
PathwayEvidenceSource
ECM proteoglycans IEA Reactome
Platelet degranulation IEA Reactome
Scavenging by Class H Receptors IEA Reactome





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