Gene description for Tpm4
Gene name tropomyosin 4
Gene symbol Tpm4
Other names/aliases Tpm4.2
Tpm4.2cy
Species Rattus norvegicus
 Database cross references - Tpm4
ExoCarta ExoCarta_24852
Entrez Gene 24852
UniProt P09495  
 Tpm4 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Tpm4
Molecular Function
    actin binding GO:0003779 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    osteoblast differentiation GO:0001649 ISO
    muscle contraction GO:0006936 TAS
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    filamentous actin GO:0031941 ISO
    membrane GO:0016020 ISO
    podosome GO:0002102 ISO
    stress fiber GO:0001725 ISO
    cytoplasm GO:0005737 ISO
    cortical cytoskeleton GO:0030863 ISO
    focal adhesion GO:0005925 ISO
 Experiment description of studies that identified Tpm4 in exosomes
1
Experiment ID 99
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Tpm4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Tpm4 is involved
PathwayEvidenceSource
Smooth Muscle Contraction IEA Reactome
Striated Muscle Contraction IEA Reactome





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