Gene description for Hspa1l
Gene name heat shock protein 1-like
Gene symbol Hspa1l
Other names/aliases Hsp70-3
Species Rattus norvegicus
 Database cross references - Hspa1l
ExoCarta ExoCarta_24963
Vesiclepedia VP_24963
Entrez Gene 24963
UniProt P55063  
 Hspa1l identified in sEVs derived from the following tissue/cell type
Hepatocytes 19367702    
 Gene ontology annotations for Hspa1l
Molecular Function
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IBA
    heat shock protein binding GO:0031072 IBA
    heat shock protein binding GO:0031072 ISO
    ubiquitin protein ligase binding GO:0031625 ISO
    protein folding chaperone GO:0044183 IBA
    unfolded protein binding GO:0051082 ISO
    ATP-dependent protein folding chaperone GO:0140662 IEA
Biological Process
    binding of sperm to zona pellucida GO:0007339 ISO
    biological_process GO:0008150 ND
    protein refolding GO:0042026 IBA
    protein refolding GO:0042026 ISO
    chaperone cofactor-dependent protein refolding GO:0051085 IBA
    positive regulation of protein targeting to mitochondrion GO:1903955 ISO
Subcellular Localization
    zona pellucida receptor complex GO:0002199 ISO
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    COP9 signalosome GO:0008180 ISO
    cell body GO:0044297 ISO
 Experiment description of studies that identified Hspa1l in sEVs
1
Experiment ID 35
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|CD81|CD63
Enriched markers
HSPA5
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Hspa1l
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml



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