Gene description for Itgam
Gene name integrin, alpha M
Gene symbol Itgam
Other names/aliases Cd11b
Species Rattus norvegicus
 Database cross references - Itgam
ExoCarta ExoCarta_25021
Vesiclepedia VP_25021
Entrez Gene 25021
 Itgam identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Itgam
Molecular Function
    opsonin binding GO:0001846 ISO
    complement component C3b binding GO:0001851 ISO
    heparin binding GO:0008201 ISO
    heat shock protein binding GO:0031072 ISO
    cargo receptor activity GO:0038024 ISO
    cargo receptor activity GO:0038024 ISO
    heparan sulfate proteoglycan binding GO:0043395 ISO
    protein-containing complex binding GO:0044877 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    microglial cell activation GO:0001774 ISO
    neutrophil apoptotic process GO:0001781 ISO
    complement receptor mediated signaling pathway GO:0002430 ISO
    leukocyte migration involved in inflammatory response GO:0002523 ISO
    response to ischemia GO:0002931 IEP
    receptor-mediated endocytosis GO:0006898 ISO
    phagocytosis GO:0006909 ISO
    phagocytosis, engulfment GO:0006911 ISO
    cell adhesion GO:0007155 ISO
    leukocyte cell-cell adhesion GO:0007159 ISO
    cell-matrix adhesion GO:0007160 ISO
    integrin-mediated signaling pathway GO:0007229 IEA
    response to mechanical stimulus GO:0009612 IEP
    ectodermal cell differentiation GO:0010668 ISO
    microglia development GO:0014005 ISO
    neutrophil chemotaxis GO:0030593 ISO
    forebrain development GO:0030900 ISO
    response to estradiol GO:0032355 IEP
    positive regulation of superoxide anion generation GO:0032930 ISO
    heterotypic cell-cell adhesion GO:0034113 IMP
    positive regulation of neutrophil degranulation GO:0043315 ISO
    cellular extravasation GO:0045123 ISO
    negative regulation of dopamine metabolic process GO:0045963 ISO
    activated T cell proliferation GO:0050798 ISO
    positive regulation of mast cell differentiation GO:0060376 ISO
    leukocyte adhesion to vascular endothelial cell GO:0061756 ISO
    positive regulation of protein targeting to membrane GO:0090314 ISO
    amyloid-beta clearance GO:0097242 ISO
    cell-cell adhesion GO:0098609 ISO
    response to Gram-positive bacterium GO:0140459 IEP
    complement-mediated synapse pruning GO:0150062 ISO
    vertebrate eye-specific patterning GO:0150064 ISO
    positive regulation of microglial cell mediated cytotoxicity GO:1904151 ISO
    response to curcumin GO:1904643 IEP
    positive regulation of prostaglandin-E synthase activity GO:2000363 ISO
Subcellular Localization
    extracellular space GO:0005615 ISO
    nucleus GO:0005634 ISO
    plasma membrane GO:0005886 ISO
    external side of plasma membrane GO:0009897 ISO
    cell surface GO:0009986 IDA
    cell surface GO:0009986 ISO
    membrane GO:0016020 ISO
    integrin alphaM-beta2 complex GO:0034688 ISO
    plasma membrane raft GO:0044853 ISO
 Experiment description of studies that identified Itgam in sEVs
1
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Itgam
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Itgam is involved
No pathways found





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