Gene description for Cyb5r3
Gene name cytochrome b5 reductase 3
Gene symbol Cyb5r3
Other names/aliases Dia1
Nadhcb5
RNNADHCB5
Species Rattus norvegicus
 Database cross references - Cyb5r3
ExoCarta ExoCarta_25035
Vesiclepedia VP_25035
Entrez Gene 25035
UniProt P20070  
 Cyb5r3 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Cyb5r3
Molecular Function
    cytochrome-b5 reductase activity, acting on NAD(P)H GO:0004128 IDA
    cytochrome-b5 reductase activity, acting on NAD(P)H GO:0004128 IEA
    cytochrome-b5 reductase activity, acting on NAD(P)H GO:0004128 ISO
    cytochrome-b5 reductase activity, acting on NAD(P)H GO:0004128 TAS
    AMP binding GO:0016208 IDA
    oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor GO:0016653 IEA
    ADP binding GO:0043531 IDA
    nitrite reductase (NO-forming) activity GO:0050421 ISO
    flavin adenine dinucleotide binding GO:0050660 IDA
    flavin adenine dinucleotide binding GO:0050660 ISO
    NAD binding GO:0051287 IDA
    FAD binding GO:0071949 IBA
    FAD binding GO:0071949 IDA
    FAD binding GO:0071949 ISO
Biological Process
    cholesterol biosynthetic process GO:0006695 IEA
    nitric oxide biosynthetic process GO:0006809 ISO
    biological_process GO:0008150 ND
Subcellular Localization
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 ISO
    mitochondrial outer membrane GO:0005741 IDA
    mitochondrial outer membrane GO:0005741 IEA
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 IEA
    endoplasmic reticulum membrane GO:0005789 ISO
    endoplasmic reticulum membrane GO:0005789 ISO
    lipid droplet GO:0005811 ISO
    cytosol GO:0005829 ISO
    mitochondrial membrane GO:0031966 ISO
    nitric-oxide synthase complex GO:1903958 ISO
 Experiment description of studies that identified Cyb5r3 in sEVs
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Cyb5r3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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