Gene description for Pc
Gene name pyruvate carboxylase
Gene symbol Pc
Other names/aliases Pcx
Species Rattus norvegicus
 Database cross references - Pc
ExoCarta ExoCarta_25104
Entrez Gene 25104
UniProt P52873  
 Pc identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Hepatocytes 19367702    
Reticulocytes 21828046    
 Gene ontology annotations for Pc
Molecular Function
    DNA binding GO:0003677 IEA
    ATP binding GO:0005524 IDA
    carboxylic acid binding GO:0031406 IDA
    pyruvate carboxylase activity GO:0004736 IMP
    metal ion binding GO:0046872 IEA
    biotin binding GO:0009374 IMP
    biotin carboxylase activity GO:0004075 IEA
Biological Process
    positive regulation of phospholipid biosynthetic process GO:0071073 IMP
    lipid metabolic process GO:0006629 IEA
    oxaloacetate metabolic process GO:0006107 IDA
    negative regulation of gene expression GO:0010629 ISO
    positive regulation by host of viral release from host cell GO:0044791 ISO
    positive regulation by host of viral process GO:0044794 ISO
    pyruvate metabolic process GO:0006090 IDA
    viral RNA genome packaging GO:0019074 ISO
    gluconeogenesis GO:0006094 IEA
Subcellular Localization
    mitochondrial inner membrane GO:0005743 ISO
    mitochondrion GO:0005739 ISO
    cytoplasm GO:0005737 ISO
    mitochondrial matrix GO:0005759 IEA
 Experiment description of studies that identified Pc in exosomes
1
Experiment ID 225
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 35
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD81|CD63
EV Membrane markers
HSPA5
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 94
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Pc
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Pc is involved
PathwayEvidenceSource
Biotin transport and metabolism IEA Reactome
Gluconeogenesis IEA Reactome





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