Gene description for Rgn
Gene name regucalcin (senescence marker protein-30)
Gene symbol Rgn
Other names/aliases GNL
Rc
Reguc
Species Rattus norvegicus
 Database cross references - Rgn
ExoCarta ExoCarta_25106
Entrez Gene 25106
UniProt Q03336  
 Rgn identified in exosomes derived from the following tissue/cell type
Hepatocytes 19367702    
Reticulocytes 21828046    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Rgn
Molecular Function
    gluconolactonase activity GO:0004341 ISS
    enzyme regulator activity GO:0030234 IEA
    zinc ion binding GO:0008270 ISS
    calcium ion binding GO:0005509 ISO
Biological Process
    positive regulation of triglyceride biosynthetic process GO:0010867 IDA
    positive regulation of superoxide dismutase activity GO:1901671 IDA
    negative regulation of DNA biosynthetic process GO:2000279 IMP
    positive regulation of ATPase activity GO:0032781 IDA
    negative regulation of sperm motility GO:1901318 IDA
    negative regulation of cyclic-nucleotide phosphodiesterase activity GO:0051344 IDA
    negative regulation of protein phosphorylation GO:0001933 IMP
    negative regulation of GTPase activity GO:0034260 IMP
    negative regulation of protein kinase activity GO:0006469 IMP
    liver regeneration GO:0097421 IEP
    negative regulation of phosphoprotein phosphatase activity GO:0032515 IMP
    negative regulation of bone development GO:1903011 IMP
    positive regulation of glucose metabolic process GO:0010907 IDA
    negative regulation of epithelial cell proliferation GO:0050680 IDA
    spermatogenesis GO:0007283 IDA
    aging GO:0007568 IEP
    negative regulation of apoptotic process GO:0043066 IDA
    positive regulation of GTPase activity GO:0043547 IDA
    positive regulation of dUTP diphosphatase activity GO:1903629 IDA
    negative regulation of nitric oxide biosynthetic process GO:0045019 IMP
    cellular calcium ion homeostasis GO:0006874 IDA
    negative regulation of DNA catabolic process GO:1903625 IDA
    negative regulation of leucine-tRNA ligase activity GO:1903634 IDA
    liver development GO:0001889 IEP
    positive regulation of phosphatase activity GO:0010922 IMP
    negative regulation of calcium-dependent ATPase activity GO:1903611 IMP
    positive regulation of fatty acid biosynthetic process GO:0045723 IDA
    negative regulation of RNA biosynthetic process GO:1902679 IMP
    regulation of calcium-mediated signaling GO:0050848 IDA
    positive regulation of calcium-transporting ATPase activity GO:1901896 IDA
    L-ascorbic acid biosynthetic process GO:0019853 ISS
    kidney development GO:0001822 IEP
    positive regulation of proteolysis involved in cellular protein catabolic process GO:1903052 IDA
Subcellular Localization
    cytosol GO:0005829 IDA
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 ISS
    nucleoplasm GO:0005654 ISO
 Experiment description of studies that identified Rgn in exosomes
1
Experiment ID 35
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD81|CD63
EV Membrane markers
HSPA5
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 192
ISEV standards
CEM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
CD63|CD81|FLOT
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M.
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Rgn
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Rgn is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here