Gene description for Gnmt
Gene name glycine N-methyltransferase
Gene symbol Gnmt
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Gnmt
ExoCarta ExoCarta_25134
Entrez Gene 25134
UniProt P13255  
 Gnmt identified in exosomes derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Gnmt
Molecular Function
    folic acid binding GO:0005542 TAS
    S-adenosylmethionine-dependent methyltransferase activity GO:0008757 IDA
    glycine N-methyltransferase activity GO:0017174 ISO
    glycine binding GO:0016594 TAS
Biological Process
    protein tetramerization GO:0051262 IDA
    methylation GO:0032259 ISO
    protein homotetramerization GO:0051289 ISO
    S-adenosylmethionine metabolic process GO:0046500 ISO
    regulation of gluconeogenesis GO:0006111 ISO
    folic acid metabolic process GO:0046655 TAS
    glycine metabolic process GO:0006544 IMP
    glycogen metabolic process GO:0005977 ISO
    methionine metabolic process GO:0006555 ISO
    one-carbon metabolic process GO:0006730 ISO
    S-adenosylhomocysteine metabolic process GO:0046498 IDA
    sarcosine metabolic process GO:1901052 IMP
Subcellular Localization
    cytosol GO:0005829 IDA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Gnmt in exosomes
1
Experiment ID 92
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
2
Experiment ID 93
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Gnmt
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Gnmt is involved
No pathways found





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