Gene description for Gnmt
Gene name glycine N-methyltransferase
Gene symbol Gnmt
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Gnmt
ExoCarta ExoCarta_25134
Vesiclepedia VP_25134
Entrez Gene 25134
UniProt P13255  
 Gnmt identified in sEVs derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Gnmt
Molecular Function
    folic acid binding GO:0005542 IDA
    folic acid binding GO:0005542 IEA
    folic acid binding GO:0005542 TAS
    S-adenosylmethionine-dependent methyltransferase activity GO:0008757 IDA
    glycine binding GO:0016594 IBA
    glycine binding GO:0016594 IDA
    glycine binding GO:0016594 IEA
    glycine binding GO:0016594 IMP
    glycine binding GO:0016594 ISO
    glycine binding GO:0016594 TAS
    glycine N-methyltransferase activity GO:0017174 IBA
    glycine N-methyltransferase activity GO:0017174 IDA
    glycine N-methyltransferase activity GO:0017174 IEA
    glycine N-methyltransferase activity GO:0017174 IMP
    glycine N-methyltransferase activity GO:0017174 ISO
    identical protein binding GO:0042802 IBA
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 IPI
    identical protein binding GO:0042802 ISO
    selenol Se-methyltransferase activity GO:0098603 TAS
    S-adenosyl-L-methionine binding GO:1904047 IBA
    S-adenosyl-L-methionine binding GO:1904047 IMP
Biological Process
    glycogen metabolic process GO:0005977 ISO
    regulation of gluconeogenesis GO:0006111 IBA
    regulation of gluconeogenesis GO:0006111 ISO
    glycine metabolic process GO:0006544 IMP
    methionine metabolic process GO:0006555 ISO
    one-carbon metabolic process GO:0006730 IBA
    one-carbon metabolic process GO:0006730 ISO
    methylation GO:0032259 IEA
    methylation GO:0032259 IMP
    S-adenosylhomocysteine metabolic process GO:0046498 IBA
    S-adenosylhomocysteine metabolic process GO:0046498 IDA
    S-adenosylhomocysteine metabolic process GO:0046498 IMP
    S-adenosylmethionine metabolic process GO:0046500 IBA
    S-adenosylmethionine metabolic process GO:0046500 IDA
    S-adenosylmethionine metabolic process GO:0046500 IEA
    S-adenosylmethionine metabolic process GO:0046500 IMP
    S-adenosylmethionine metabolic process GO:0046500 ISO
    protein homotetramerization GO:0051289 IBA
    protein homotetramerization GO:0051289 IEA
    protein homotetramerization GO:0051289 IPI
    protein homotetramerization GO:0051289 ISO
    sarcosine metabolic process GO:1901052 IBA
    sarcosine metabolic process GO:1901052 IMP
Subcellular Localization
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    methyltransferase complex GO:0034708 IMP
 Experiment description of studies that identified Gnmt in sEVs
1
Experiment ID 92
MISEV standards
EM
Biophysical techniques
HSP70|GAPDH|CD9|CD151
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
2
Experiment ID 93
MISEV standards
EM
Biophysical techniques
HSP70|GAPDH|CD9|CD151
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Gnmt
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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