ExoCarta: Gene summary

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Gene description for Tapbp
Gene name	TAP binding protein (tapasin)
Gene symbol	Tapbp
Other names/aliases	-
Species	Rattus norvegicus
Database cross references - Tapbp
ExoCarta	ExoCarta_25217
Vesiclepedia	VP_25217
Entrez Gene	25217
Tapbp identified in sEVs derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Tapbp

Molecular Function
	MHC class I protein complex binding	GO:0023024	IBA
	protein folding chaperone	GO:0044183	ISO
	TAP1 binding	GO:0046978	ISO
	TAP2 binding	GO:0046979	ISO
	unfolded protein binding	GO:0051082	NAS
	molecular adaptor activity	GO:0060090	ISO
	TAP complex binding	GO:0062061	IBA
	TAP complex binding	GO:0062061	ISO
Biological Process
	MHC class I protein complex assembly	GO:0002397	IEP
	MHC class Ib protein complex assembly	GO:0002398	ISO
	antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent	GO:0002479	ISO
	antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent	GO:0002479	ISO
	peptide antigen assembly with MHC class I protein complex	GO:0002502	IBA
	peptide antigen assembly with MHC class I protein complex	GO:0002502	ISO
	protein folding	GO:0006457	IEA
	defense response	GO:0006952	ISO
	regulation of gene expression	GO:0010468	ISO
	antigen processing and presentation of endogenous peptide antigen via MHC class I	GO:0019885	IEA
	peptide antigen stabilization	GO:0050823	ISO
	regulation of protein complex stability	GO:0061635	ISO
	protein-containing complex assembly	GO:0065003	ISO
Subcellular Localization
	Golgi membrane	GO:0000139	ISO
	endoplasmic reticulum membrane	GO:0005789	ISO
	MHC class I peptide loading complex	GO:0042824	IBA
	MHC class I peptide loading complex	GO:0042824	IDA
	MHC class I peptide loading complex	GO:0042824	ISO
	Tapasin-ERp57 complex	GO:0061779	ISO

Experiment description of studies that identified Tapbp in sEVs

Experiment ID

MISEV standards
✔ EM	Biophysical techniques
✔ HSC70\|HSP90\|TSG101\|MHCI	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

"Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D2 Rep 1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.16-1.21 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting