Gene description for Aqp1
Gene name aquaporin 1
Gene symbol Aqp1
Other names/aliases CHIP28
Species Rattus norvegicus
 Database cross references - Aqp1
ExoCarta ExoCarta_25240
Entrez Gene 25240
UniProt P29975  
 Aqp1 identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Aqp1
Molecular Function
    water channel activity GO:0015250 ISO
    protein binding GO:0005515 IPI
    transmembrane transporter activity GO:0022857 ISO
    ammonium transmembrane transporter activity GO:0008519 ISS
    intracellular cGMP activated cation channel activity GO:0005223 ISS
    potassium channel activity GO:0005267 ISS
    glycerol transmembrane transporter activity GO:0015168 ISO
    carbon dioxide transmembrane transporter activity GO:0035379 ISS
    nitric oxide transmembrane transporter activity GO:0030184 ISS
    water transmembrane transporter activity GO:0005372 ISO
    ephrin receptor binding GO:0046875 ISO
    glycerol channel activity GO:0015254 IBA
    potassium ion transmembrane transporter activity GO:0015079 ISO
Biological Process
    wound healing GO:0042060 ISO
    cellular response to mercury ion GO:0071288 ISS
    cerebrospinal fluid secretion GO:0033326 ISO
    hyperosmotic response GO:0006972 IEP
    cellular response to UV GO:0034644 ISS
    sensory perception of pain GO:0019233 ISO
    positive regulation of fibroblast proliferation GO:0048146 ISS
    positive regulation of epithelial cell migration GO:0010634 IMP
    cellular response to inorganic substance GO:0071241 ISS
    cellular response to copper ion GO:0071280 ISS
    cellular homeostasis GO:0019725 ISS
    carbon dioxide transmembrane transport GO:0035378 ISS
    response to drug GO:0042493 ISS
    negative regulation of apoptotic process GO:0043066 ISS
    glomerular filtration GO:0003094 ISO
    multicellular organismal water homeostasis GO:0050891 ISO
    glycerol transport GO:0015793 ISO
    cellular response to cAMP GO:0071320 ISS
    camera-type eye morphogenesis GO:0048593 ISO
    potassium ion transmembrane transport GO:0071805 ISS
    cation transmembrane transport GO:0098655 ISO
    cellular response to mechanical stimulus GO:0071260 ISS
    cell volume homeostasis GO:0006884 ISS
    nitric oxide transport GO:0030185 ISS
    cellular response to hydrogen peroxide GO:0070301 ISS
    cellular water homeostasis GO:0009992 IBA
    secretion by cell GO:0032940 ISO
    ammonium transport GO:0015696 ISS
    water transport GO:0006833 ISO
    maintenance of symbiont-containing vacuole by host GO:0085018 ISS
    renal water absorption GO:0070295 ISO
    cGMP biosynthetic process GO:0006182 ISS
    response to estrogen GO:0043627 IEP
    positive regulation of angiogenesis GO:0045766 ISS
    metanephric glomerulus vasculature development GO:0072239 IEA
    cellular response to hypoxia GO:0071456 ISS
    cellular response to salt stress GO:0071472 ISS
    pancreatic juice secretion GO:0030157 ISO
    water homeostasis GO:0030104 ISO
    cellular response to dexamethasone stimulus GO:0071549 ISS
    response to hormone GO:0009725 IEP
    secretory granule organization GO:0033363 ISO
    lipid digestion GO:0044241 ISO
    metanephric proximal convoluted tubule segment 2 development GO:0072232 IEA
    lateral ventricle development GO:0021670 ISO
    metanephric descending thin limb development GO:0072220 IEA
    positive regulation of lamellipodium assembly GO:0010592 IMP
    transepithelial water transport GO:0035377 ISS
    establishment or maintenance of actin cytoskeleton polarity GO:0030950 ISS
    ammonium transmembrane transport GO:0072488 ISO
    corticotropin secretion GO:0051458 ISO
    potassium ion transport GO:0006813 ISO
    carbon dioxide transport GO:0015670 ISS
    renal water transport GO:0003097 ISS
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 ISO
    positive regulation of cell migration GO:0030335 ISO
    cellular response to retinoic acid GO:0071300 ISS
    cellular hyperosmotic response GO:0071474 ISS
    metanephric proximal straight tubule development GO:0072230 IEA
    positive regulation of saliva secretion GO:0046878 ISS
    cellular response to nitric oxide GO:0071732 ISO
    odontogenesis GO:0042476 ISO
    hyperosmotic salinity response GO:0042538 IEP
Subcellular Localization
    sarcolemma GO:0042383 ISS
    apical part of cell GO:0045177 ISO
    neuron projection GO:0043005 IDA
    integral component of membrane GO:0016021 ISO
    symbiont-containing vacuole membrane GO:0020005 ISO
    basolateral plasma membrane GO:0016323 ISO
    brush border membrane GO:0031526 ISS
    symbiont-containing vacuole GO:0020003 ISO
    axon terminus GO:0043679 IDA
    nuclear membrane GO:0031965 ISS
    neuronal cell body membrane GO:0032809 IDA
    cytoplasm GO:0005737 ISS
    brush border GO:0005903 ISO
    membrane GO:0016020 ISO
    cell GO:0005623 ISO
    integral component of plasma membrane GO:0005887 IBA
    plasma membrane GO:0005886 ISO
    basal plasma membrane GO:0009925 ISS
    extracellular exosome GO:0070062 ISO
    nucleus GO:0005634 ISS
    axon GO:0030424 ISO
    apical plasma membrane GO:0016324 ISO
 Experiment description of studies that identified Aqp1 in exosomes
1
Experiment ID 225
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 90
ISEV standards
EM
EV Biophysical techniques
HSC70|HSP90|TSG101
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 94
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
8
Experiment ID 99
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
9
Experiment ID 100
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
10
Experiment ID 101
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
11
Experiment ID 192
ISEV standards
CEM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
CD63|CD81|FLOT
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M.
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Aqp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here