Gene description for Tst
Gene name thiosulfate sulfurtransferase
Gene symbol Tst
Other names/aliases RHODAN
Species Rattus norvegicus
 Database cross references - Tst
ExoCarta ExoCarta_25274
Vesiclepedia VP_25274
Entrez Gene 25274
UniProt P24329  
 Tst identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Hepatocytes 19367702    
 Gene ontology annotations for Tst
Molecular Function
    thiosulfate sulfurtransferase activity GO:0004792 IBA
    thiosulfate sulfurtransferase activity GO:0004792 IEA
    5S rRNA binding GO:0008097 IEA
    5S rRNA binding GO:0008097 ISO
    5S rRNA binding GO:0008097 ISS
    sulfurtransferase activity GO:0016783 TAS
    3-mercaptopyruvate sulfurtransferase activity GO:0016784 IBA
Biological Process
    iron-sulfur cluster assembly GO:0016226 NAS
    epithelial cell differentiation GO:0030855 IEA
    epithelial cell differentiation GO:0030855 ISO
    rRNA import into mitochondrion GO:0035928 IEA
    rRNA import into mitochondrion GO:0035928 ISO
    rRNA import into mitochondrion GO:0035928 ISS
    rRNA transport GO:0051029 ISO
    rRNA transport GO:0051029 ISS
Subcellular Localization
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 ISO
    mitochondrial matrix GO:0005759 IEA
    mitochondrial matrix GO:0005759 TAS
 Experiment description of studies that identified Tst in sEVs
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 35
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|CD81|CD63
Enriched markers
HSPA5
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Tst
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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