Gene description for Mfge8
Gene name milk fat globule-EGF factor 8 protein
Gene symbol Mfge8
Other names/aliases AGS
MFGME8
MFGMP-E8
OAcGD3S
Species Rattus norvegicus
 Database cross references - Mfge8
ExoCarta ExoCarta_25277
Entrez Gene 25277
UniProt P70490  
 Mfge8 identified in exosomes derived from the following tissue/cell type
Dendritic cells 19812188    
Hepatocytes 19367702    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Mfge8
Molecular Function
    phosphatidylethanolamine binding GO:0008429 ISO
    phosphatidylserine binding GO:0001786 ISO
    integrin binding GO:0005178 ISO
Biological Process
    phagocytosis, engulfment GO:0006911 ISO
    response to estrogen GO:0043627 IEP
    angiogenesis GO:0001525 IEA
    phagocytosis, recognition GO:0006910 ISO
    positive regulation of apoptotic cell clearance GO:2000427 ISO
    positive regulation of cell proliferation GO:0008284 IEP
    positive regulation of phagocytosis GO:0050766 ISO
    cell adhesion GO:0007155 IEA
    single fertilization GO:0007338 IEA
Subcellular Localization
    extracellular vesicle GO:1903561 ISO
    membrane GO:0016020 ISO
    extracellular region GO:0005576 IEA
    extracellular space GO:0005615 ISO
    extracellular matrix GO:0031012 ISO
    extrinsic component of plasma membrane GO:0019897 ISO
    extracellular exosome GO:0070062 ISO
    external side of plasma membrane GO:0009897 ISO
 Experiment description of studies that identified Mfge8 in exosomes
1
Experiment ID 69
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19812188    
Organism Rattus norvegicus
Experiment description Immature dendritic cell-derived exosomes rescue septic animals via milk fat globule epidermal growth factor VIII.
Authors Miksa M, Wu R, Dong W, Komura H, Amin D, Ji Y, Wang Z, Wang H, Ravikumar TS, Tracey KJ, Wang P
Journal name JIMMU
Publication year 2009
Sample Dendritic cells
Sample name Bone marrow-derived immature dendritic cells
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
2
Experiment ID 35
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD81|CD63
EV Membrane markers
HSPA5
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 93
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Mfge8
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Mfge8 is involved
No pathways found





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