Experiment description of studies that identified Mfge8 in exosomes

1 Experiment ID 69 ISEV standards ✘ EV Biophysical techniques ✘ EV Cytosolic markers ✘ EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 19812188     Organism Rattus norvegicus Experiment description Immature dendritic cell-derived exosomes rescue septic animals via milk fat globule epidermal growth factor VIII. Authors Miksa M, Wu R, Dong W, Komura H, Amin D, Ji Y, Wang Z, Wang H, Ravikumar TS, Tracey KJ, Wang P Journal name JIMMU Publication year 2009 Sample Dendritic cells Sample name Bone marrow-derived immature dendritic cells Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.15 g/mL Molecules identified in the study Protein Methods used in the study Western blotting

2 Experiment ID 35 ISEV standards ✔ CEM EV Biophysical techniques ✔ TSG101|Alix EV Cytosolic markers ✔ CD81|CD63 EV Membrane markers ✔ HSPA5 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Western blotting Mass spectrometry PubMed ID 19367702     Organism Rattus norvegicus Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes. Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM Journal name JPR Publication year 2008 Sample Hepatocytes Sample name Hepatocytes Isolation/purification methods Differential centrifugation Filtration Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [QTOF] Western blotting

3 Experiment ID 93 ISEV standards ✔ EM EV Biophysical techniques ✔ HSP70|GAPDH EV Cytosolic markers ✔ CD9|CD151 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry [MALDI TOF] PubMed ID 20124479     Organism Rattus norvegicus Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation. Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M. Journal name CR Publication year 2010 Sample Pancreatic cancer cells Sample name AS-Tspan8 Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.14-1.17 g/mL Molecules identified in the study Protein mRNA Methods used in the study RT-PCR Western blotting

Protein-protein interactions for Mfge8

Protein Interactor ExoCarta ID Identification method PubMed Species No interactions are found.

Pathways in which Mfge8 is involved

No pathways found