Gene description for Alox5
Gene name arachidonate 5-lipoxygenase
Gene symbol Alox5
Other names/aliases LOX5A
Species Rattus norvegicus
 Database cross references - Alox5
ExoCarta ExoCarta_25290
Entrez Gene 25290
UniProt P12527  
 Alox5 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Alox5
Molecular Function
    arachidonate 5-lipoxygenase activity GO:0004051 ISO
    protein binding GO:0005515 IPI
    iron ion binding GO:0005506 ISS
Biological Process
    acute inflammatory response GO:0002526 IMP
    sensory perception of pain GO:0019233 IMP
    positive regulation of vasoconstriction GO:0045907 IMP
    lipoxygenase pathway GO:0019372 IMP
    response to nutrient GO:0007584 IEP
    oxidation-reduction process GO:0055114 IEA
    inflammatory response GO:0006954 ISO
    leukotriene metabolic process GO:0006691 ISO
    arachidonic acid metabolic process GO:0019369 IMP
    response to hyperoxia GO:0055093 IEP
    leukotriene biosynthetic process GO:0019370 TAS
    leukotriene production involved in inflammatory response GO:0002540 ISO
Subcellular Localization
    nuclear envelope lumen GO:0005641 ISS
    dendrite GO:0030425 IDA
    nuclear envelope GO:0005635 TAS
    sarcolemma GO:0042383 IDA
    nuclear matrix GO:0016363 ISO
    nuclear membrane GO:0031965 ISS
    nucleus GO:0005634 ISO
    cytosol GO:0005829 ISS
    cytoplasm GO:0005737 ISO
    extracellular space GO:0005615 ISO
 Experiment description of studies that identified Alox5 in exosomes
1
Experiment ID 97
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Alox5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Alox5 is involved
No pathways found





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