Gene description for Ephx1
Gene name epoxide hydrolase 1, microsomal (xenobiotic)
Gene symbol Ephx1
Other names/aliases MEH8
Species Rattus norvegicus
 Database cross references - Ephx1
ExoCarta ExoCarta_25315
Entrez Gene 25315
UniProt P07687  
 Ephx1 identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Hepatocytes 19367702    
 Gene ontology annotations for Ephx1
Molecular Function
    cis-stilbene-oxide hydrolase activity GO:0033961 IEA
    epoxide hydrolase activity GO:0004301 IDA
    enzyme binding GO:0019899 IPI
Biological Process
    cellular response to glucocorticoid stimulus GO:0071385 IEP
    cellular aromatic compound metabolic process GO:0006725 ISO
    aromatic compound catabolic process GO:0019439 IEA
    response to toxic substance GO:0009636 IEA
    liver development GO:0001889 IEP
    cellular response to organic substance GO:0071310 IEP
    xenobiotic metabolic process GO:0006805 TAS
    response to organic cyclic compound GO:0014070 ISO
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 IEA
    integral component of membrane GO:0016021 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified Ephx1 in exosomes
1
Experiment ID 225
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 35
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD81|CD63
EV Membrane markers
HSPA5
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Ephx1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ephx1 is involved
No pathways found





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