Gene description for Ephx1
Gene name epoxide hydrolase 1, microsomal (xenobiotic)
Gene symbol Ephx1
Other names/aliases MEH8
Species Rattus norvegicus
 Database cross references - Ephx1
ExoCarta ExoCarta_25315
Vesiclepedia VP_25315
Entrez Gene 25315
UniProt P07687  
 Ephx1 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Hepatocytes 19367702    
 Gene ontology annotations for Ephx1
Molecular Function
    epoxide hydrolase activity GO:0004301 IBA
    epoxide hydrolase activity GO:0004301 IDA
    epoxide hydrolase activity GO:0004301 IMP
    epoxide hydrolase activity GO:0004301 ISO
    oxysterol binding GO:0008142 IEA
    oxysterol binding GO:0008142 ISO
    oxysterol binding GO:0008142 ISS
    enzyme binding GO:0019899 IPI
    cis-stilbene-oxide hydrolase activity GO:0033961 IEA
    cis-stilbene-oxide hydrolase activity GO:0033961 IMP
    cis-stilbene-oxide hydrolase activity GO:0033961 ISO
    cis-stilbene-oxide hydrolase activity GO:0033961 ISS
Biological Process
    liver development GO:0001889 IEP
    lipid metabolic process GO:0006629 IEA
    catabolic process GO:0009056 IEA
    response to toxic substance GO:0009636 IEA
    arachidonate metabolic process GO:0019369 IBA
    arachidonate metabolic process GO:0019369 ISO
    arachidonate metabolic process GO:0019369 ISS
    diol biosynthetic process GO:0034312 IDA
    cellular response to glucocorticoid stimulus GO:0071385 IEP
    epoxide metabolic process GO:0097176 IBA
    epoxide metabolic process GO:0097176 IDA
    epoxide metabolic process GO:0097176 ISO
    hydrocarbon catabolic process GO:0120253 IEA
    hydrocarbon catabolic process GO:0120253 ISO
    response to polycyclic arene GO:1903165 IEA
    response to polycyclic arene GO:1903165 ISO
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 IEA
    membrane GO:0016020 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified Ephx1 in sEVs
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 35
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|CD81|CD63
Enriched markers
HSPA5
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Ephx1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ephx1 is involved
PathwayEvidenceSource
Biological oxidations IEA Reactome
Metabolism IEA Reactome
Phase I - Functionalization of compounds IEA Reactome





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