Gene description for Adk
Gene name adenosine kinase
Gene symbol Adk
Other names/aliases AK
Species Rattus norvegicus
 Database cross references - Adk
ExoCarta ExoCarta_25368
Entrez Gene 25368
UniProt Q64640  
 Adk identified in exosomes derived from the following tissue/cell type
Hepatocytes 19367702    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Adk
Molecular Function
    poly(A) RNA binding GO:0044822 ISO
    metal ion binding GO:0046872 IEA
    ATP binding GO:0005524 IEA
    phosphotransferase activity, alcohol group as acceptor GO:0016773 IEA
    adenosine kinase activity GO:0004001 ISO
Biological Process
    purine ribonucleoside salvage GO:0006166 NAS
    dATP biosynthetic process GO:0006175 ISO
    positive regulation of cardiac muscle hypertrophy GO:0010613 IMP
    positive regulation of T cell proliferation GO:0042102 IMP
    adenosine metabolic process GO:0046085 IDA
    circadian regulation of gene expression GO:0032922 IEP
    phosphorylation GO:0016310 IEA
    type B pancreatic cell proliferation GO:0044342 IMP
    AMP salvage GO:0044209 IEA
Subcellular Localization
    nucleoplasm GO:0005654 ISO
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Adk in exosomes
1
Experiment ID 35
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD81|CD63
EV Membrane markers
✔
HSPA5
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 95
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Adk
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Adk is involved
PathwayEvidenceSource
Purine salvage IEA Reactome





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