Gene description for Adk
Gene name adenosine kinase
Gene symbol Adk
Other names/aliases AK
Species Rattus norvegicus
 Database cross references - Adk
ExoCarta ExoCarta_25368
Vesiclepedia VP_25368
Entrez Gene 25368
UniProt Q64640  
 Adk identified in sEVs derived from the following tissue/cell type
Hepatocytes 19367702    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Adk
Molecular Function
    adenosine kinase activity GO:0004001 IBA
    adenosine kinase activity GO:0004001 IDA
    adenosine kinase activity GO:0004001 IEA
    adenosine kinase activity GO:0004001 ISO
    deoxyadenosine kinase activity GO:0004136 IEA
    deoxyadenosine kinase activity GO:0004136 ISO
    ATP binding GO:0005524 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    purine nucleobase metabolic process GO:0006144 IBA
    purine ribonucleoside salvage GO:0006166 IEA
    purine ribonucleoside salvage GO:0006166 ISO
    purine ribonucleoside salvage GO:0006166 NAS
    dATP biosynthetic process GO:0006175 IEA
    dATP biosynthetic process GO:0006175 ISO
    positive regulation of cardiac muscle hypertrophy GO:0010613 IMP
    GMP salvage GO:0032263 IEA
    GMP salvage GO:0032263 ISO
    circadian regulation of gene expression GO:0032922 IEP
    positive regulation of T cell proliferation GO:0042102 IEP
    positive regulation of T cell proliferation GO:0042102 IMP
    AMP salvage GO:0044209 IEA
    AMP salvage GO:0044209 ISO
    type B pancreatic cell proliferation GO:0044342 IMP
    adenosine metabolic process GO:0046085 IDA
    dAMP salvage GO:0106383 IEA
    dAMP salvage GO:0106383 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
 Experiment description of studies that identified Adk in sEVs
1
Experiment ID 35
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|CD81|CD63
Enriched markers
HSPA5
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Adk
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Adk is involved
PathwayEvidenceSource
Drug ADME IEA Reactome
Metabolism IEA Reactome
Metabolism of nucleotides IEA Reactome
Nucleotide salvage IEA Reactome
Purine salvage IEA Reactome
Ribavirin ADME IEA Reactome





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