Gene description for Alad
Gene name aminolevulinate dehydratase
Gene symbol Alad
Other names/aliases ALADR
aminolevulinatedelta-dehydratase
Species Rattus norvegicus
 Database cross references - Alad
ExoCarta ExoCarta_25374
Vesiclepedia VP_25374
Entrez Gene 25374
UniProt P06214  
 Alad identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Alad
Molecular Function
    porphobilinogen synthase activity GO:0004655 IBA
    porphobilinogen synthase activity GO:0004655 IDA
    porphobilinogen synthase activity GO:0004655 IEA
    porphobilinogen synthase activity GO:0004655 ISO
    porphobilinogen synthase activity GO:0004655 ISS
    zinc ion binding GO:0008270 IBA
    zinc ion binding GO:0008270 ISO
    zinc ion binding GO:0008270 ISS
    identical protein binding GO:0042802 ISO
    metal ion binding GO:0046872 IEA
    proteasome core complex binding GO:1904854 IDA
Biological Process
    response to hypoxia GO:0001666 IEP
    protoporphyrinogen IX biosynthetic process GO:0006782 IEA
    heme biosynthetic process GO:0006783 IBA
    heme biosynthetic process GO:0006783 IDA
    heme biosynthetic process GO:0006783 ISO
    heme biosynthetic process GO:0006783 ISO
    heme biosynthetic process GO:0006783 ISS
    heme A biosynthetic process GO:0006784 ISO
    heme B biosynthetic process GO:0006785 ISO
    response to oxidative stress GO:0006979 IEP
    response to nutrient GO:0007584 IDA
    response to xenobiotic stimulus GO:0009410 IDA
    response to xenobiotic stimulus GO:0009410 IEP
    response to herbicide GO:0009635 IEP
    response to toxic substance GO:0009636 IEP
    response to hormone GO:0009725 IEP
    response to metal ion GO:0010038 IDA
    response to metal ion GO:0010038 IEP
    response to iron ion GO:0010039 IEP
    response to zinc ion GO:0010043 IDA
    response to aluminum ion GO:0010044 IEP
    response to ionizing radiation GO:0010212 IEP
    response to vitamin B1 GO:0010266 IEP
    response to selenium ion GO:0010269 IEP
    response to lead ion GO:0010288 IEP
    response to organic cyclic compound GO:0014070 IEP
    response to activity GO:0014823 IEP
    response to nutrient levels GO:0031667 IEP
    response to cobalt ion GO:0032025 IEP
    response to lipopolysaccharide GO:0032496 IEP
    response to vitamin E GO:0033197 IEP
    response to vitamin GO:0033273 IEP
    response to amino acid GO:0043200 IEP
    response to ethanol GO:0045471 IDA
    response to arsenic-containing substance GO:0046685 IDA
    response to arsenic-containing substance GO:0046685 IEP
    response to cadmium ion GO:0046686 IDA
    response to cadmium ion GO:0046686 IEP
    response to mercury ion GO:0046689 IEP
    heme O biosynthetic process GO:0048034 ISO
    protein homooligomerization GO:0051260 ISO
    response to glucocorticoid GO:0051384 IEP
    response to methylmercury GO:0051597 IEP
    response to platinum ion GO:0070541 IEP
    response to fatty acid GO:0070542 IEP
    cellular response to lead ion GO:0071284 IEP
    cellular response to interleukin-4 GO:0071353 ISO
    negative regulation of proteasomal protein catabolic process GO:1901799 ISO
Subcellular Localization
    extracellular space GO:0005615 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Alad in sEVs
1
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 99
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Alad
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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