Gene description for Anxa1
Gene name annexin A1
Gene symbol Anxa1
Other names/aliases Anx1
p35
Species Rattus norvegicus
 Database cross references - Anxa1
ExoCarta ExoCarta_25380
Entrez Gene 25380
UniProt P07150  
 Anxa1 identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Anxa1
Molecular Function
    calcium-dependent protein binding GO:0048306 ISO
    protein homodimerization activity GO:0042803 IDA
    double-stranded DNA-dependent ATPase activity GO:0033676 IMP
    single-stranded DNA binding GO:0003697 IDA
    calcium-dependent phospholipid binding GO:0005544 TAS
    phospholipase A2 inhibitor activity GO:0019834 ISO
    helicase activity GO:0004386 IMP
    single-stranded RNA binding GO:0003727 IDA
    protein binding, bridging GO:0030674 ISO
    structural molecule activity GO:0005198 ISO
    phospholipid binding GO:0005543 IDA
    annealing helicase activity GO:0036310 IMP
    calcium ion binding GO:0005509 IEA
Biological Process
    DNA rewinding GO:0036292 IMP
    positive regulation of vesicle fusion GO:0031340 ISO
    DNA strand renaturation GO:0000733 IMP
    negative regulation of interleukin-8 secretion GO:2000483 ISO
    regulation of cell proliferation GO:0042127 ISO
    prolactin secretion GO:0070459 IMP
    positive regulation of prostaglandin biosynthetic process GO:0031394 IMP
    DNA duplex unwinding GO:0032508 IMP
    response to corticosteroid GO:0031960 IEP
    cell surface receptor signaling pathway GO:0007166 IDA
    cellular response to hydrogen peroxide GO:0070301 IMP
    positive regulation of apoptotic process GO:0043065 IMP
    negative regulation of phospholipase A2 activity GO:1900138 IDA
    estrous cycle GO:0044849 IEP
    gliogenesis GO:0042063 IEP
    insulin secretion GO:0030073 IDA
    response to estradiol GO:0032355 IEP
    arachidonic acid secretion GO:0050482 ISO
    endocrine pancreas development GO:0031018 IEP
    peptide cross-linking GO:0018149 ISO
    negative regulation of protein secretion GO:0050709 IMP
    neutrophil homeostasis GO:0001780 ISO
    response to organic cyclic compound GO:0014070 IEP
    alpha-beta T cell differentiation GO:0046632 ISO
    positive regulation of neutrophil apoptotic process GO:0033031 ISO
    neutrophil clearance GO:0097350 ISO
    positive regulation of G1/S transition of mitotic cell cycle GO:1900087 ISO
    response to drug GO:0042493 IEP
    cellular response to glucocorticoid stimulus GO:0071385 ISO
    response to glucocorticoid GO:0051384 IEP
    response to X-ray GO:0010165 IEP
    hepatocyte differentiation GO:0070365 IEP
    keratinocyte differentiation GO:0030216 ISO
    signal transduction GO:0007165 ISO
    response to peptide hormone GO:0043434 IEP
    prostate gland development GO:0030850 IEP
    response to interleukin-1 GO:0070555 IEP
    response to hormone GO:0009725 IEP
Subcellular Localization
    cytoplasm GO:0005737 ISO
    extracellular space GO:0005615 ISO
    extracellular exosome GO:0070062 ISO
    mast cell granule GO:0042629 IDA
    sarcolemma GO:0042383 ISO
    endosome GO:0005768 ISO
    cornified envelope GO:0001533 ISO
    nucleus GO:0005634 ISO
    focal adhesion GO:0005925 ISO
    vesicle GO:0031982 ISO
    basolateral plasma membrane GO:0016323 IEA
    plasma membrane GO:0005886 ISO
    cell surface GO:0009986 ISO
    cilium GO:0005929 IEA
    mitochondrial membrane GO:0031966 IDA
    protein complex GO:0043234 IDA
 Experiment description of studies that identified Anxa1 in exosomes
1
Experiment ID 225
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 90
ISEV standards
EM
EV Biophysical techniques
HSC70|HSP90|TSG101
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 94
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Anxa1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here