Gene description for Eno3
Gene name enolase 3, beta, muscle
Gene symbol Eno3
Other names/aliases BBE
MSE
Species Rattus norvegicus
 Database cross references - Eno3
ExoCarta ExoCarta_25438
Vesiclepedia VP_25438
Entrez Gene 25438
UniProt P15429  
 Eno3 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Eno3
Molecular Function
    magnesium ion binding GO:0000287 IEA
    phosphopyruvate hydratase activity GO:0004634 IBA
    phosphopyruvate hydratase activity GO:0004634 IDA
    phosphopyruvate hydratase activity GO:0004634 IEA
    phosphopyruvate hydratase activity GO:0004634 ISO
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    protein-containing complex binding GO:0044877 IDA
Biological Process
    glycolytic process GO:0006096 IBA
    glycolytic process GO:0006096 IEA
    response to xenobiotic stimulus GO:0009410 IEP
    substantia nigra development GO:0021762 ISO
    skeletal muscle tissue regeneration GO:0043403 IEP
    canonical glycolysis GO:0061621 IEA
    canonical glycolysis GO:0061621 ISO
Subcellular Localization
    phosphopyruvate hydratase complex GO:0000015 IBA
    phosphopyruvate hydratase complex GO:0000015 IEA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    membrane GO:0016020 ISO
 Experiment description of studies that identified Eno3 in sEVs
1
Experiment ID 99
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Eno3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Eno3 is involved
PathwayEvidenceSource
Gluconeogenesis IEA Reactome
Glucose metabolism IEA Reactome
Glycolysis IEA Reactome
Metabolism IEA Reactome
Metabolism of carbohydrates IEA Reactome





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