Gene description for Lypla1
Gene name lysophospholipase I
Gene symbol Lypla1
Other names/aliases 25KDL
Pla1a
Species Rattus norvegicus
 Database cross references - Lypla1
ExoCarta ExoCarta_25514
Entrez Gene 25514
UniProt P70470  
 Lypla1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Lypla1
Molecular Function
    lysophospholipase activity GO:0004622 IDA
    palmitoyl-(protein) hydrolase activity GO:0008474 ISS
    lipase activity GO:0016298 ISO
Biological Process
    fatty acid metabolic process GO:0006631 IEA
    macromolecule depalmitoylation GO:0098734 ISS
    protein depalmitoylation GO:0002084 ISO
    negative regulation of Golgi to plasma membrane protein transport GO:0042997 ISO
Subcellular Localization
    cytosol GO:0005829 ISO
    mitochondrion GO:0005739 ISO
    extracellular exosome GO:0070062 ISO
    cytoplasm GO:0005737 IEA
 Experiment description of studies that identified Lypla1 in exosomes
1
Experiment ID 96
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Lypla1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Lypla1 is involved
PathwayEvidenceSource
eNOS activation IEA Reactome





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