Gene description for Prkag1
Gene name protein kinase, AMP-activated, gamma 1 non-catalytic subunit
Gene symbol Prkag1
Other names/aliases Prkaac
Prkga1
Species Rattus norvegicus
 Database cross references - Prkag1
ExoCarta ExoCarta_25520
Vesiclepedia VP_25520
Entrez Gene 25520
UniProt P80385  
 Prkag1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Prkag1
Molecular Function
    protein kinase activity GO:0004672 IEA
    AMP-activated protein kinase activity GO:0004679 IDA
    AMP-activated protein kinase activity GO:0004679 IMP
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    AMP binding GO:0016208 IBA
    AMP binding GO:0016208 IDA
    AMP binding GO:0016208 IMP
    protein kinase regulator activity GO:0019887 IBA
    protein kinase regulator activity GO:0019887 IDA
    protein kinase regulator activity GO:0019887 IEA
    protein kinase regulator activity GO:0019887 ISO
    protein kinase binding GO:0019901 IBA
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 IPI
    protein kinase binding GO:0019901 ISO
    ADP binding GO:0043531 IDA
    protein-containing complex binding GO:0044877 IDA
Biological Process
    regulation of glycolytic process GO:0006110 IBA
    fatty acid biosynthetic process GO:0006633 IEA
    positive regulation of gene expression GO:0010628 IEA
    cellular response to nutrient levels GO:0031669 IEA
    cellular response to nutrient levels GO:0031669 ISO
    cellular response to glucose starvation GO:0042149 IBA
    regulation of carbon utilization GO:0043609 IBA
    positive regulation of gluconeogenesis GO:0045722 IBA
    regulation of catalytic activity GO:0050790 IDA
    import into nucleus GO:0051170 IEA
    import into nucleus GO:0051170 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 TAS
    nucleotide-activated protein kinase complex GO:0031588 IBA
    nucleotide-activated protein kinase complex GO:0031588 IDA
    nucleotide-activated protein kinase complex GO:0031588 IEA
    nucleotide-activated protein kinase complex GO:0031588 ISO
    protein-containing complex GO:0032991 IDA
 Experiment description of studies that identified Prkag1 in sEVs
1
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Prkag1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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