Gene description for Rab4a
Gene name RAB4A, member RAS oncogene family
Gene symbol Rab4a
Other names/aliases Rab4
Species Rattus norvegicus
 Database cross references - Rab4a
ExoCarta ExoCarta_25532
Vesiclepedia VP_25532
Entrez Gene 25532
UniProt P05714  
 Rab4a identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Rab4a
Molecular Function
    ATPase activator activity GO:0001671 IDA
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IDA
    GTPase activity GO:0003924 IEA
    GTPase activity GO:0003924 ISO
    GTPase activity GO:0003924 ISS
    G protein activity GO:0003925 IEA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IDA
    GTP binding GO:0005525 IEA
    GTP binding GO:0005525 ISO
    GTP binding GO:0005525 ISS
    GTP binding GO:0005525 TAS
    GDP binding GO:0019003 ISO
    GDP binding GO:0019003 ISS
    syntaxin binding GO:0019905 IPI
    ionotropic glutamate receptor binding GO:0035255 IPI
    ATPase binding GO:0051117 IPI
Biological Process
    small GTPase-mediated signal transduction GO:0007264 ISO
    protein transport GO:0015031 IEA
    protein transport GO:0015031 ISO
    vesicle-mediated transport GO:0016192 IBA
    antigen processing and presentation GO:0019882 ISO
    regulation of endocytosis GO:0030100 IBA
    regulation of endocytosis GO:0030100 IEA
    regulation of endocytosis GO:0030100 ISO
    Rab protein signal transduction GO:0032482 IC
    Rab protein signal transduction GO:0032482 IEA
    Rab protein signal transduction GO:0032482 IMP
    neurotransmitter receptor transport postsynaptic membrane to endosome GO:0098968 IEA
    neurotransmitter receptor transport postsynaptic membrane to endosome GO:0098968 ISO
    vesicle-mediated transport in synapse GO:0099003 IEA
    vesicle-mediated transport in synapse GO:0099003 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    endosome GO:0005768 ISO
    plasma membrane GO:0005886 IEA
    synaptic vesicle membrane GO:0030672 IDA
    synaptic vesicle membrane GO:0030672 IEA
    early endosome membrane GO:0031901 IEA
    vesicle GO:0031982 ISO
    insulin-responsive compartment GO:0032593 IBA
    insulin-responsive compartment GO:0032593 IDA
    perinuclear region of cytoplasm GO:0048471 ISO
    recycling endosome GO:0055037 IDA
    recycling endosome membrane GO:0055038 IEA
    extracellular exosome GO:0070062 ISO
    postsynaptic recycling endosome GO:0098837 IBA
    glutamatergic synapse GO:0098978 IEA
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Rab4a in sEVs
1
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Rab4a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here