ExoCarta: Gene summary

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Gene description for Rab4a
Gene name	RAB4A, member RAS oncogene family
Gene symbol	Rab4a
Other names/aliases	Rab4
Species	Rattus norvegicus
Database cross references - Rab4a
ExoCarta	ExoCarta_25532
Entrez Gene	25532
UniProt	P05714
Rab4a identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Rab4a

Molecular Function
	ATPase activator activity	GO:0001671	IDA
	protein transporter activity	GO:0008565	ISO
	GTP binding	GO:0005525	TAS
	GDP binding	GO:0019003	ISS
	protein binding	GO:0005515	IPI
	ATPase binding	GO:0051117	IDA
	ionotropic glutamate receptor binding	GO:0035255	IPI
	syntaxin binding	GO:0019905	IPI
	GTPase activity	GO:0003924	ISS
Biological Process
	positive regulation of ATPase activity	GO:0032781	IDA
	antigen processing and presentation	GO:0019882	ISO
	regulation of endocytosis	GO:0030100	ISO
	small GTPase mediated signal transduction	GO:0007264	IEA
	intracellular protein transport	GO:0006886	IBA
	metabolic process	GO:0008152	ISS
	protein transport	GO:0015031	ISO
	Rab protein signal transduction	GO:0032482	IMP
Subcellular Localization
	cytosol	GO:0005829	IDA
	membrane	GO:0016020	IDA
	recycling endosome	GO:0055037	IDA
	plasma membrane	GO:0005886	IDA
	cytoplasm	GO:0005737	ISO
	intracellular membrane-bounded organelle	GO:0043231	IDA
	perinuclear region of cytoplasm	GO:0048471	ISO
	endosome	GO:0005768	ISO
	insulin-responsive compartment	GO:0032593	IDA
	vesicle	GO:0031982	ISO
	extracellular exosome	GO:0070062	ISO

Experiment description of studies that identified Rab4a in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ HSC70\|HSP90\|TSG101	EV Cytosolic markers
✔ MHCI	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D2 Rep 1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.16-1.21 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting