Gene description for Map3k12
Gene name mitogen activated protein kinase kinase kinase 12
Gene symbol Map3k12
Other names/aliases DLK
MUK
PK
Zpk
Species Rattus norvegicus
 Database cross references - Map3k12
ExoCarta ExoCarta_25579
Vesiclepedia VP_25579
Entrez Gene 25579
UniProt Q63796  
 Map3k12 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
 Gene ontology annotations for Map3k12
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 ISO
    protein serine/threonine kinase activity GO:0004674 ISS
    JUN kinase kinase kinase activity GO:0004706 IEA
    MAP kinase kinase kinase activity GO:0004709 IEA
    ATP binding GO:0005524 IEA
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    protein homodimerization activity GO:0042803 IEA
    protein homodimerization activity GO:0042803 ISO
    protein homodimerization activity GO:0042803 ISS
    protein serine/threonine kinase activator activity GO:0043539 IEA
    protein serine/threonine kinase activator activity GO:0043539 ISO
    protein serine kinase activity GO:0106310 IEA
Biological Process
    protein phosphorylation GO:0006468 ISO
    JNK cascade GO:0007254 IBA
    JNK cascade GO:0007254 IEA
    JNK cascade GO:0007254 ISO
    peptidyl-serine phosphorylation GO:0018105 ISO
    peptidyl-threonine phosphorylation GO:0018107 ISO
    positive regulation of JUN kinase activity GO:0043507 ISO
    post-translational protein modification GO:0043687 IEA
    post-translational protein modification GO:0043687 ISO
    post-translational protein modification GO:0043687 ISS
    positive regulation of protein kinase activity GO:0045860 ISO
    positive regulation of DNA-templated transcription GO:0045893 IEA
    positive regulation of DNA-templated transcription GO:0045893 ISO
    positive regulation of DNA-templated transcription GO:0045893 ISS
    protein autophosphorylation GO:0046777 ISO
    protein autophosphorylation GO:0046777 ISS
    regulation of macromolecule metabolic process GO:0060255 IEA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IEA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISO
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISS
    regulation of primary metabolic process GO:0080090 IEA
    negative regulation of motor neuron apoptotic process GO:2000672 IEA
    negative regulation of motor neuron apoptotic process GO:2000672 ISO
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    axon GO:0030424 ISO
    growth cone GO:0030426 IEA
    growth cone GO:0030426 ISO
 Experiment description of studies that identified Map3k12 in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for Map3k12
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Map3k12 is involved
No pathways found





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