Gene description for Pcmt1
Gene name protein-L-isoaspartate (D-aspartate) O-methyltransferase 1
Gene symbol Pcmt1
Other names/aliases PCM
Species Rattus norvegicus
 Database cross references - Pcmt1
ExoCarta ExoCarta_25604
Vesiclepedia VP_25604
Entrez Gene 25604
UniProt P22062  
 Pcmt1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Pcmt1
Molecular Function
    protein-L-isoaspartate (D-aspartate) O-methyltransferase activity GO:0004719 IBA
    protein-L-isoaspartate (D-aspartate) O-methyltransferase activity GO:0004719 IDA
    protein-L-isoaspartate (D-aspartate) O-methyltransferase activity GO:0004719 IEA
    protein-L-isoaspartate (D-aspartate) O-methyltransferase activity GO:0004719 ISO
    protein binding GO:0005515 IPI
    S-adenosylmethionine-dependent methyltransferase activity GO:0008757 IDA
Biological Process
    protein methylation GO:0006479 IDA
    protein methylation GO:0006479 ISO
    protein methylation GO:0006479 ISO
    negative regulation of cardiac muscle cell apoptotic process GO:0010667 IMP
    methylation GO:0032259 IEA
    protein modification process GO:0036211 IEA
    response to ethanol GO:0045471 IEP
    S-adenosylhomocysteine metabolic process GO:0046498 IDA
    S-adenosylmethionine metabolic process GO:0046500 IDA
    cellular response to hypoxia GO:0071456 IEP
Subcellular Localization
    extracellular space GO:0005615 IDA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    basolateral plasma membrane GO:0016323 IDA
    brush border membrane GO:0031526 IDA
    perikaryon GO:0043204 IDA
 Experiment description of studies that identified Pcmt1 in sEVs
1
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Pcmt1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Pcmt1 is involved
PathwayEvidenceSource
Metabolism of proteins IEA Reactome
Protein repair IEA Reactome





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