Gene description for Gckr
Gene name glucokinase (hexokinase 4) regulator
Gene symbol Gckr
Other names/aliases GLRE
Species Rattus norvegicus
 Database cross references - Gckr
ExoCarta ExoCarta_25658
Vesiclepedia VP_25658
Entrez Gene 25658
UniProt Q07071  
 Gckr identified in exosomes derived from the following tissue/cell type
Hepatocytes 19367702    
 Gene ontology annotations for Gckr
Molecular Function
    protein domain specific binding GO:0019904 IPI
    enzyme binding GO:0019899 IPI
    carbohydrate binding GO:0030246 IMP
    fructose-6-phosphate binding GO:0070095 ISS
    protein binding GO:0005515 IPI
    enzyme inhibitor activity GO:0004857 IDA
Biological Process
    triglyceride homeostasis GO:0070328 ISO
    protein localization to nucleus GO:0034504 ISO
    positive regulation of glucokinase activity GO:0033133 ISO
    urate metabolic process GO:0046415 ISO
    negative regulation of glucokinase activity GO:0033132 ISO
    response to fructose GO:0009750 ISO
    negative regulation of catalytic activity GO:0043086 IDA
    protein import into nucleus, translocation GO:0000060 ISO
    cellular glucose homeostasis GO:0001678 ISO
    glucose metabolic process GO:0006006 TAS
    detection of glucose GO:0051594 NAS
Subcellular Localization
    mitochondrion GO:0005739 IDA
    intracellular GO:0005622 ISO
    cytoplasm GO:0005737 IDA
    cell GO:0005623 ISO
    nucleoplasm GO:0005654 IDA
    nucleus GO:0005634 ISO
    cytosol GO:0005829 IDA
 Experiment description of studies that identified Gckr in exosomes
1
Experiment ID 35
MISEV standards
CEM
EV Biophysical techniques
TSG101|Alix|CD81|CD63
EV Enriched markers
HSPA5
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Gckr
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Gckr is involved
PathwayEvidenceSource
Regulation of Glucokinase by Glucokinase Regulatory Protein IEA Reactome





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