Gene description for Gnai1
Gene name guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1
Gene symbol Gnai1
Other names/aliases BPGTPB
Species Rattus norvegicus
 Database cross references - Gnai1
ExoCarta ExoCarta_25686
Vesiclepedia VP_25686
Entrez Gene 25686
UniProt P10824  
 Gnai1 identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
 Gene ontology annotations for Gnai1
Molecular Function
    magnesium ion binding GO:0000287 IDA
    magnesium ion binding GO:0000287 IMP
    G protein-coupled receptor binding GO:0001664 IDA
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IDA
    GTPase activity GO:0003924 IMP
    GTPase activity GO:0003924 ISO
    GTPase activity GO:0003924 TAS
    G protein activity GO:0003925 IEA
    G protein activity GO:0003925 ISO
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IDA
    GTP binding GO:0005525 IEA
    GTP binding GO:0005525 IMP
    GTP binding GO:0005525 ISO
    adenylate cyclase regulator activity GO:0010854 IEA
    adenylate cyclase regulator activity GO:0010854 ISO
    GDP binding GO:0019003 IDA
    GDP binding GO:0019003 IMP
    G-protein beta/gamma-subunit complex binding GO:0031683 IBA
    G-protein beta/gamma-subunit complex binding GO:0031683 IEA
    D2 dopamine receptor binding GO:0031749 IEA
    D2 dopamine receptor binding GO:0031749 ISO
    G protein-coupled serotonin receptor binding GO:0031821 IBA
    G protein-coupled serotonin receptor binding GO:0031821 IPI
    GTPase activating protein binding GO:0032794 IDA
    metal ion binding GO:0046872 IEA
Biological Process
    G protein-coupled receptor signaling pathway GO:0007186 IDA
    G protein-coupled receptor signaling pathway GO:0007186 IMP
    adenylate cyclase-modulating G protein-coupled receptor signaling pathway GO:0007188 IBA
    adenylate cyclase-modulating G protein-coupled receptor signaling pathway GO:0007188 IMP
    adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway GO:0007193 ISO
    adenylate cyclase-inhibiting serotonin receptor signaling pathway GO:0007198 IEA
    adenylate cyclase-inhibiting serotonin receptor signaling pathway GO:0007198 ISO
    response to prostaglandin E GO:0034695 IEA
    response to prostaglandin E GO:0034695 ISO
    positive regulation of cholesterol biosynthetic process GO:0045542 IEA
    positive regulation of cholesterol biosynthetic process GO:0045542 ISO
    negative regulation of synaptic transmission GO:0050805 IMP
    cell division GO:0051301 IEA
    cell division GO:0051301 ISO
    cell division GO:0051301 ISS
    regulation of mitotic spindle organization GO:0060236 IEA
    regulation of mitotic spindle organization GO:0060236 ISO
    regulation of mitotic spindle organization GO:0060236 ISS
    T cell migration GO:0072678 IEA
    T cell migration GO:0072678 ISO
    neurotransmitter receptor localization to postsynaptic specialization membrane GO:0099645 IDA
    neurotransmitter receptor localization to postsynaptic specialization membrane GO:0099645 IEP
    cellular response to forskolin GO:1904322 IMP
    positive regulation of protein localization to cell cortex GO:1904778 IEA
    positive regulation of protein localization to cell cortex GO:1904778 ISO
    positive regulation of protein localization to cell cortex GO:1904778 ISS
Subcellular Localization
    nucleus GO:0005634 IEA
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    centrosome GO:0005813 IDA
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    heterotrimeric G-protein complex GO:0005834 IBA
    heterotrimeric G-protein complex GO:0005834 IEA
    heterotrimeric G-protein complex GO:0005834 ISO
    heterotrimeric G-protein complex GO:0005834 TAS
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 TAS
    cell cortex GO:0005938 IEA
    cell cortex GO:0005938 ISO
    cell cortex GO:0005938 ISS
    midbody GO:0030496 IEA
    midbody GO:0030496 ISO
    midbody GO:0030496 ISS
    protein-containing complex GO:0032991 IDA
    postsynapse GO:0098794 IDA
    postsynapse GO:0098794 NAS
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 NAS
 Experiment description of studies that identified Gnai1 in exosomes
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for Gnai1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
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