Gene description for Gnai1
Gene name guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1
Gene symbol Gnai1
Other names/aliases BPGTPB
Species Rattus norvegicus
 Database cross references - Gnai1
ExoCarta ExoCarta_25686
Entrez Gene 25686
UniProt P10824  
 Gnai1 identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
 Gene ontology annotations for Gnai1
Molecular Function
    GTP binding GO:0005525 IEA
    GDP binding GO:0019003 IDA
    GTPase activating protein binding GO:0032794 IDA
    G-protein beta/gamma-subunit complex binding GO:0031683 IEA
    metal ion binding GO:0046872 IEA
    protein binding GO:0005515 IPI
    GTPase activity GO:0003924 IEA
    G-protein coupled serotonin receptor binding GO:0031821 IPI
    signal transducer activity GO:0004871 IEA
Biological Process
    metabolic process GO:0008152 IEA
    cell division GO:0051301 ISS
    adenylate cyclase-modulating G-protein coupled receptor signaling pathway GO:0007188 IEA
    negative regulation of synaptic transmission GO:0050805 IMP
    G-protein coupled receptor signaling pathway GO:0007186 IMP
Subcellular Localization
    cytoplasm GO:0005737 ISO
    plasma membrane GO:0005886 TAS
    midbody GO:0030496 ISS
    heterotrimeric G-protein complex GO:0005834 TAS
    membrane raft GO:0045121 IDA
    intracellular GO:0005622 ISO
    lysosomal membrane GO:0005765 ISO
    protein complex GO:0043234 IDA
    centrosome GO:0005813 ISO
    membrane GO:0016020 IDA
    nucleus GO:0005634 IEA
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Gnai1 in exosomes
1
Experiment ID 225
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for Gnai1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Gnai1 is involved
PathwayEvidenceSource
G alpha (i) signalling events IEA Reactome
G alpha (z) signalling events IEA Reactome





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