Gene description for Ass1
Gene name argininosuccinate synthase 1
Gene symbol Ass1
Other names/aliases ASSA
Ass
Species Rattus norvegicus
 Database cross references - Ass1
ExoCarta ExoCarta_25698
Entrez Gene 25698
UniProt P09034  
 Ass1 identified in exosomes derived from the following tissue/cell type
Hepatocytes 19367702    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Ass1
Molecular Function
    amino acid binding GO:0016597 ISO
    ATP binding GO:0005524 IEA
    argininosuccinate synthase activity GO:0004055 ISO
    toxic substance binding GO:0015643 IPI
    poly(A) RNA binding GO:0044822 ISO
    identical protein binding GO:0042802 ISO
Biological Process
    positive regulation of nitric oxide biosynthetic process GO:0045429 ISO
    response to fatty acid GO:0070542 IEP
    argininosuccinate metabolic process GO:0000053 ISO
    aging GO:0007568 IEP
    cellular response to ammonium ion GO:0071242 IEP
    response to estradiol GO:0032355 IEP
    response to amino acid GO:0043200 IEP
    response to peptide hormone GO:0043434 IEP
    urea cycle GO:0000050 ISO
    arginine biosynthetic process GO:0006526 IEA
    kidney development GO:0001822 IEP
    liver development GO:0001889 IEP
    response to amine GO:0014075 IEP
    negative regulation of leukocyte cell-cell adhesion GO:1903038 ISO
    cellular response to oleic acid GO:0071400 IEP
    midgut development GO:0007494 IEP
    cellular response to dexamethasone stimulus GO:0071549 IEP
    cellular response to tumor necrosis factor GO:0071356 IEP
    aspartate metabolic process GO:0006531 ISO
    cellular response to amine stimulus GO:0071418 IEP
    response to toxic substance GO:0009636 IEP
    cellular response to glucagon stimulus GO:0071377 IEP
    diaphragm development GO:0060539 IEP
    citrulline metabolic process GO:0000052 ISO
    response to lipopolysaccharide GO:0032496 IEP
    cellular response to lipopolysaccharide GO:0071222 IEP
    cellular response to amino acid stimulus GO:0071230 IEP
    response to drug GO:0042493 IEP
    response to zinc ion GO:0010043 IDA
    response to glucocorticoid GO:0051384 IDA
    cellular response to laminar fluid shear stress GO:0071499 ISO
    cellular response to interferon-gamma GO:0071346 IEP
    response to growth hormone GO:0060416 IEP
    cellular response to cAMP GO:0071320 IEP
    response to steroid hormone GO:0048545 IEP
    acute-phase response GO:0006953 IEP
    response to mycotoxin GO:0010046 IDA
    response to nutrient GO:0007584 IEP
Subcellular Localization
    mitochondrial outer membrane GO:0005741 IDA
    neuron projection GO:0043005 IDA
    neuronal cell body GO:0043025 IDA
    myelin sheath GO:0043209 ISO
    cell body fiber GO:0070852 IDA
    cytoplasm GO:0005737 IDA
    lysosome GO:0005764 IDA
    nucleus GO:0005634 IDA
    extracellular exosome GO:0070062 ISO
    endoplasmic reticulum GO:0005783 IDA
    perikaryon GO:0043204 IDA
    mitochondrion GO:0005739 ISO
 Experiment description of studies that identified Ass1 in exosomes
1
Experiment ID 35
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD81|CD63
EV Membrane markers
HSPA5
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ass1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ass1 is involved
PathwayEvidenceSource
Urea cycle IEA Reactome





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