ExoCarta: Gene summary

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Gene description for Ide
Gene name	insulin degrading enzyme
Gene symbol	Ide
Other names/aliases	INSDEGM
Species	Rattus norvegicus
Database cross references - Ide
ExoCarta	ExoCarta_25700
Entrez Gene	25700
UniProt	P35559
Ide identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Ide

Molecular Function
	beta-amyloid binding	GO:0001540	IPI
	peptide hormone binding	GO:0017046	IPI
	peptide binding	GO:0042277	ISO
	zinc ion binding	GO:0008270	ISS
	protein homodimerization activity	GO:0042803	ISO
	glycoprotein binding	GO:0001948	ISO
	ubiquitin binding	GO:0043130	ISO
	receptor binding	GO:0005102	ISO
	ATPase activity	GO:0016887	IDA
	beta-endorphin binding	GO:0031626	IMP
	insulin binding	GO:0043559	ISO
	ATP binding	GO:0005524	ISS
	metalloendopeptidase activity	GO:0004222	ISS
	peptidase activity	GO:0008233	TAS
Biological Process
	cellular protein catabolic process	GO:0044257	IDA
	negative regulation of proteolysis	GO:0045861	IDA
	insulin catabolic process	GO:1901143	ISO
	proteolysis	GO:0006508	ISO
	protein homooligomerization	GO:0051260	ISO
	peptide catabolic process	GO:0043171	TAS
	proteolysis involved in cellular protein catabolic process	GO:0051603	ISO
	determination of adult lifespan	GO:0008340	ISO
	positive regulation of protein oligomerization	GO:0032461	ISO
	beta-amyloid metabolic process	GO:0050435	ISS
	ubiquitin homeostasis	GO:0010992	ISO
	protein homotetramerization	GO:0051289	IDA
	bradykinin catabolic process	GO:0010815	ISS
	hormone catabolic process	GO:0042447	IDA
	insulin metabolic process	GO:1901142	ISO
	protein heterooligomerization	GO:0051291	IDA
Subcellular Localization
	cytosol	GO:0005829	ISO
	nucleoplasm	GO:0005654	ISO
	cytosolic proteasome complex	GO:0031597	IDA
	mitochondrion	GO:0005739	ISO
	peroxisome	GO:0005777	ISO
	plasma membrane	GO:0005886	IEA
	cell surface	GO:0009986	ISO
	cytoplasm	GO:0005737	ISO
	nucleus	GO:0005634	ISO
	extracellular space	GO:0005615	ISO
	peroxisomal matrix	GO:0005782	IDA

Experiment description of studies that identified Ide in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ TSG101\|HSP90\|HSC70	EV Cytosolic markers
✔ MHCI	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D7 Rep 1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.13-1.25 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting