Gene description for Prkar1a
Gene name protein kinase, cAMP-dependent, regulatory, type I, alpha
Gene symbol Prkar1a
Other names/aliases RIIA
Species Rattus norvegicus
 Database cross references - Prkar1a
ExoCarta ExoCarta_25725
Entrez Gene 25725
UniProt P09456  
 Prkar1a identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Prkar1a
Molecular Function
    cAMP binding GO:0030552 IDA
    ubiquitin protein ligase binding GO:0031625 ISO
    protein kinase A catalytic subunit binding GO:0034236 ISO
    cAMP-dependent protein kinase inhibitor activity GO:0004862 ISO
    cAMP-dependent protein kinase regulator activity GO:0008603 TAS
Biological Process
    mesoderm formation GO:0001707 ISO
    regulation of protein kinase activity GO:0045859 ISO
    negative regulation of meiotic nuclear division GO:0045835 IEP
    female meiotic division GO:0007143 IEP
    cardiac muscle cell proliferation GO:0060038 ISO
    sarcomere organization GO:0045214 ISO
    negative regulation of cAMP-dependent protein kinase activity GO:2000480 ISO
    heart development GO:0007507 ISO
    negative regulation of protein kinase activity GO:0006469 ISO
Subcellular Localization
    cAMP-dependent protein kinase complex GO:0005952 IDA
    cytoplasm GO:0005737 IDA
    neuromuscular junction GO:0031594 ISO
    plasma membrane GO:0005886 IEA
    protein complex GO:0043234 ISO
    AMP-activated protein kinase complex GO:0031588 ISO
    membrane GO:0016020 ISO
 Experiment description of studies that identified Prkar1a in exosomes
1
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Prkar1a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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