Gene description for Ogt
Gene name O-linked N-acetylglucosamine (GlcNAc) transferase
Gene symbol Ogt
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Ogt
ExoCarta ExoCarta_26295
Entrez Gene 26295
UniProt P56558  
 Ogt identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Ogt
Molecular Function
    protein O-GlcNAc transferase activity GO:0097363 ISS
    protein binding GO:0005515 IPI
    peptide binding GO:0042277 IDA
    transcription factor binding GO:0008134 IPI
    histone acetyltransferase activity (H4-K8 specific) GO:0043996 ISS
    histone acetyltransferase activity (H4-K16 specific) GO:0046972 ISS
    protein N-acetylglucosaminyltransferase activity GO:0016262 ISO
    histone acetyltransferase activity (H4-K5 specific) GO:0043995 ISS
    monosaccharide binding GO:0048029 IDA
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 ISS
    protein domain specific binding GO:0019904 IPI
    enzyme activator activity GO:0008047 ISO
Biological Process
    cellular response to insulin stimulus GO:0032869 IEP
    positive regulation of histone H3-K27 methylation GO:0061087 ISS
    negative regulation of peptidyl-threonine phosphorylation GO:0010801 IMP
    negative regulation of cellular response to hypoxia GO:1900038 IMP
    cellular response to retinoic acid GO:0071300 ISO
    regulation of insulin receptor signaling pathway GO:0046626 ISO
    protein heterotrimerization GO:0070208 IDA
    positive regulation of proteolysis GO:0045862 ISS
    histone H4-K5 acetylation GO:0043981 ISS
    forebrain development GO:0030900 IEP
    positive regulation of reactive oxygen species biosynthetic process GO:1903428 IMP
    protein homotrimerization GO:0070207 IDA
    histone H4-K16 acetylation GO:0043984 ISS
    positive regulation of protein phosphorylation GO:0001934 IMP
    positive regulation of granulocyte differentiation GO:0030854 ISO
    positive regulation of gene expression GO:0010628 IMP
    negative regulation of cell death GO:0060548 IMP
    cellular response to toxic substance GO:0097237 IEP
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISS
    regulation of Rac protein signal transduction GO:0035020 ISO
    histone H4-K8 acetylation GO:0043982 ISS
    cellular response to lipopolysaccharide GO:0071222 IMP
    protein O-linked glycosylation GO:0006493 ISO
    cellular response to glucose stimulus GO:0071333 IMP
    positive regulation of catalytic activity GO:0043085 ISO
    regulation of gluconeogenesis involved in cellular glucose homeostasis GO:0090526 ISS
    glucosamine metabolic process GO:0006041 IMP
    negative regulation of peptidyl-serine phosphorylation GO:0033137 IMP
    intracellular distribution of mitochondria GO:0048312 IMP
    positive regulation of protein localization to nucleus GO:1900182 IMP
    negative regulation of protein phosphorylation GO:0001933 IMP
    response to insulin GO:0032868 ISO
    negative regulation of protein targeting to membrane GO:0090315 IMP
    positive regulation of histone H3-K4 methylation GO:0051571 ISO
    circadian regulation of gene expression GO:0032922 ISS
    regulation of glycolytic process GO:0006110 ISS
    positive regulation of cell size GO:0045793 IMP
    negative regulation of protein ubiquitination GO:0031397 ISS
    apoptotic process GO:0006915 ISS
    histone H3-K4 trimethylation GO:0080182 ISS
    phosphatidylinositol-mediated signaling GO:0048015 ISS
Subcellular Localization
    neuronal cell body GO:0043025 IDA
    euchromatin GO:0000791 IDA
    mitochondrion GO:0005739 IDA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 ISS
    microtubule organizing center GO:0005815 ISO
    histone acetyltransferase complex GO:0000123 ISS
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    MLL5-L complex GO:0070688 ISO
    zymogen granule GO:0042588 IDA
    neuron projection GO:0043005 IDA
 Experiment description of studies that identified Ogt in exosomes
1
Experiment ID 90
ISEV standards
EM
EV Biophysical techniques
HSC70|HSP90|TSG101
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ogt
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ogt is involved
No pathways found





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