Gene description for Ogt
Gene name O-linked N-acetylglucosamine (GlcNAc) transferase
Gene symbol Ogt
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Ogt
ExoCarta ExoCarta_26295
Vesiclepedia VP_26295
Entrez Gene 26295
UniProt P56558  
 Ogt identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Ogt
Molecular Function
    protein binding GO:0005515 IPI
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 IEA
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 ISO
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 ISS
    glycosyltransferase activity GO:0016757 IEA
    protein domain specific binding GO:0019904 IPI
    chromatin DNA binding GO:0031490 IEA
    chromatin DNA binding GO:0031490 ISO
    peptide binding GO:0042277 IDA
    identical protein binding GO:0042802 IPI
    protein-containing complex binding GO:0044877 IDA
    protein O-acetylglucosaminyltransferase activity GO:0097363 IBA
    protein O-acetylglucosaminyltransferase activity GO:0097363 IDA
    protein O-acetylglucosaminyltransferase activity GO:0097363 ISO
    protein O-acetylglucosaminyltransferase activity GO:0097363 ISS
    RNA polymerase II C-terminal domain S5 O-GlcNAc transferase activity GO:0140842 IEA
    RNA polymerase II C-terminal domain S7 O-GlcNAc transferase activity GO:0140843 IEA
Biological Process
    mitophagy GO:0000423 IEA
    mitophagy GO:0000423 ISO
    positive regulation of transcription from RNA polymerase II promoter by glucose GO:0000432 IEA
    positive regulation of transcription from RNA polymerase II promoter by glucose GO:0000432 ISO
    negative regulation of protein phosphorylation GO:0001933 IMP
    positive regulation of protein phosphorylation GO:0001934 IMP
    glucosamine metabolic process GO:0006041 IDA
    glucosamine metabolic process GO:0006041 IMP
    regulation of glycolytic process GO:0006110 IEA
    regulation of glycolytic process GO:0006110 ISO
    regulation of glycolytic process GO:0006110 ISS
    regulation of gluconeogenesis GO:0006111 IEA
    regulation of gluconeogenesis GO:0006111 ISO
    regulation of gluconeogenesis GO:0006111 ISS
    chromatin organization GO:0006325 IEA
    regulation of transcription by RNA polymerase II GO:0006357 ISO
    protein O-linked glycosylation GO:0006493 IBA
    protein O-linked glycosylation GO:0006493 IDA
    protein O-linked glycosylation GO:0006493 IEA
    protein O-linked glycosylation GO:0006493 IMP
    protein O-linked glycosylation GO:0006493 ISO
    protein O-linked glycosylation GO:0006493 ISO
    apoptotic process GO:0006915 IEA
    apoptotic process GO:0006915 ISO
    apoptotic process GO:0006915 ISS
    positive regulation of gene expression GO:0010628 IMP
    negative regulation of peptidyl-threonine phosphorylation GO:0010801 IMP
    protein processing GO:0016485 IEA
    protein processing GO:0016485 ISO
    protein processing GO:0016485 ISS
    hemopoiesis GO:0030097 IEA
    hemopoiesis GO:0030097 ISO
    forebrain development GO:0030900 IEP
    negative regulation of protein ubiquitination GO:0031397 IEA
    negative regulation of protein ubiquitination GO:0031397 ISO
    negative regulation of protein ubiquitination GO:0031397 ISS
    negative regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032435 IEA
    negative regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032435 ISO
    response to insulin GO:0032868 IEA
    response to insulin GO:0032868 ISO
    cellular response to insulin stimulus GO:0032869 IEP
    circadian regulation of gene expression GO:0032922 IEA
    circadian regulation of gene expression GO:0032922 ISO
    circadian regulation of gene expression GO:0032922 ISS
    negative regulation of peptidyl-serine phosphorylation GO:0033137 IMP
    regulation of Rac protein signal transduction GO:0035020 IEA
    regulation of Rac protein signal transduction GO:0035020 ISO
    positive regulation of translation GO:0045727 IEA
    positive regulation of translation GO:0045727 ISO
    positive regulation of cell size GO:0045793 IMP
    positive regulation of proteolysis GO:0045862 IEA
    positive regulation of proteolysis GO:0045862 ISO
    positive regulation of proteolysis GO:0045862 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    regulation of insulin receptor signaling pathway GO:0046626 IEA
    regulation of insulin receptor signaling pathway GO:0046626 ISO
    positive regulation of lipid biosynthetic process GO:0046889 IEA
    positive regulation of lipid biosynthetic process GO:0046889 ISO
    intracellular distribution of mitochondria GO:0048312 IMP
    regulation of synapse assembly GO:0051963 IEA
    regulation of synapse assembly GO:0051963 ISO
    cellular response to lipopolysaccharide GO:0071222 IMP
    cellular response to glucose stimulus GO:0071333 IEA
    cellular response to glucose stimulus GO:0071333 IMP
    cellular response to glucose stimulus GO:0071333 ISO
    cellular response to glucose stimulus GO:0071333 ISS
    negative regulation of protein targeting to membrane GO:0090315 IMP
    cellular response to toxic substance GO:0097237 IEP
    regulation of neurotransmitter receptor localization to postsynaptic specialization membrane GO:0098696 IEA
    regulation of neurotransmitter receptor localization to postsynaptic specialization membrane GO:0098696 ISO
    positive regulation of cold-induced thermogenesis GO:0120162 ISO
    positive regulation of cold-induced thermogenesis GO:0120162 ISS
    negative regulation of non-canonical inflammasome complex assembly GO:0160076 IEA
    negative regulation of non-canonical inflammasome complex assembly GO:0160076 ISO
    negative regulation of non-canonical inflammasome complex assembly GO:0160076 ISS
    negative regulation of cellular response to hypoxia GO:1900038 IMP
    positive regulation of protein localization to nucleus GO:1900182 IMP
    positive regulation of reactive oxygen species biosynthetic process GO:1903428 IMP
    positive regulation of TORC1 signaling GO:1904263 IEA
    positive regulation of TORC1 signaling GO:1904263 ISO
    positive regulation of TORC1 signaling GO:1904263 ISS
Subcellular Localization
    histone acetyltransferase complex GO:0000123 ISO
    histone acetyltransferase complex GO:0000123 ISS
    euchromatin GO:0000791 IDA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    protein N-acetylglucosaminyltransferase complex GO:0017122 IDA
    protein N-acetylglucosaminyltransferase complex GO:0017122 IEA
    protein N-acetylglucosaminyltransferase complex GO:0017122 ISO
    mitochondrial membrane GO:0031966 IEA
    protein-containing complex GO:0032991 ISO
    zymogen granule GO:0042588 IDA
    cell projection GO:0042995 IEA
    neuronal cell body GO:0043025 IDA
    NSL complex GO:0044545 IEA
    NSL complex GO:0044545 ISO
    synapse GO:0045202 ISO
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IEA
    glutamatergic synapse GO:0098978 ISO
    GABA-ergic synapse GO:0098982 IDA
    presynaptic cytosol GO:0099523 IDA
    postsynaptic cytosol GO:0099524 IDA
    cerebellar granule cell to Purkinje cell synapse GO:0150048 IDA
 Experiment description of studies that identified Ogt in sEVs
1
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ogt
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Dao  
Affinity Capture-MS Rattus norvegicus
2 Trak2  
Affinity Capture-Western Rattus norvegicus
Two-hybrid Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
3 Atxn10  
Reconstituted Complex Rattus norvegicus
Co-fractionation Rattus norvegicus
Reconstituted Complex Rattus norvegicus
4 Foxo4  
Affinity Capture-Western Rattus norvegicus
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