Gene description for Plekhg3
Gene name pleckstrin homology domain containing, family G (with RhoGef domain) member 3
Gene symbol Plekhg3
Other names/aliases BC030417
Species Mus musculus
 Database cross references - Plekhg3
ExoCarta ExoCarta_263406
Vesiclepedia VP_263406
Entrez Gene 263406
UniProt Q4VAC9  
 Plekhg3 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Plekhg3
Molecular Function
    actin binding GO:0003779 ISO
    actin binding GO:0003779 ISS
    guanyl-nucleotide exchange factor activity GO:0005085 IBA
    guanyl-nucleotide exchange factor activity GO:0005085 IEA
    small GTPase binding GO:0031267 IBA
Biological Process
    regulation of cell migration GO:0030334 ISO
    regulation of cell migration GO:0030334 ISS
    regulation of establishment of cell polarity GO:2000114 IBA
    regulation of establishment of cell polarity GO:2000114 ISO
    regulation of establishment of cell polarity GO:2000114 ISS
Subcellular Localization
    cellular_component GO:0005575 ND
    cytoplasm GO:0005737 IEA
    cytoskeleton GO:0005856 IEA
 Experiment description of studies that identified Plekhg3 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Plekhg3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Eed  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



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