Gene description for Dapp1
Gene name dual adaptor for phosphotyrosine and 3-phosphoinositides 1
Gene symbol Dapp1
Other names/aliases Bam32
Species Mus musculus
 Database cross references - Dapp1
ExoCarta ExoCarta_26377
Vesiclepedia VP_26377
Entrez Gene 26377
UniProt Q9QXT1  
 Dapp1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Dapp1
Molecular Function
    phospholipid binding GO:0005543 IBA
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 IEA
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 ISO
    phosphatidylinositol-3,4-bisphosphate binding GO:0043325 IBA
    phosphatidylinositol-3,4-bisphosphate binding GO:0043325 IEA
    phosphatidylinositol-3,4-bisphosphate binding GO:0043325 ISO
Subcellular Localization
    cytoplasm GO:0005737 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
 Experiment description of studies that identified Dapp1 in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dapp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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