ExoCarta: Gene summary

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Gene description for Acot7
Gene name	acyl-CoA thioesterase 7
Gene symbol	Acot7
Other names/aliases	Bach
Species	Rattus norvegicus
Database cross references - Acot7
ExoCarta	ExoCarta_26759
Vesiclepedia	VP_26759
Entrez Gene	26759
UniProt	Q64559
Acot7 identified in sEVs derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Acot7

Molecular Function
	fatty-acyl-CoA binding	GO:0000062	ISO
	thiolester hydrolase activity	GO:0016790	IEA
	long-chain fatty acyl-CoA binding	GO:0036042	IEA
	long-chain fatty acyl-CoA binding	GO:0036042	ISO
	identical protein binding	GO:0042802	ISO
	protein homodimerization activity	GO:0042803	IEA
	protein homodimerization activity	GO:0042803	ISO
	fatty acyl-CoA hydrolase activity	GO:0047617	IDA
	carboxylic ester hydrolase activity	GO:0052689	IEA
	long-chain fatty acyl-CoA hydrolase activity	GO:0052816	IBA
	long-chain fatty acyl-CoA hydrolase activity	GO:0052816	IEA
	long-chain fatty acyl-CoA hydrolase activity	GO:0052816	ISO
Biological Process
	fatty acid metabolic process	GO:0006631	IEA
	acyl-CoA metabolic process	GO:0006637	IBA
	fatty acid catabolic process	GO:0009062	IBA
	fatty acid catabolic process	GO:0009062	ISO
	coenzyme A biosynthetic process	GO:0015937	IEA
	coenzyme A biosynthetic process	GO:0015937	ISO
	medium-chain fatty-acyl-CoA catabolic process	GO:0036114	IEA
	medium-chain fatty-acyl-CoA catabolic process	GO:0036114	ISO
	long-chain fatty-acyl-CoA catabolic process	GO:0036116	IEA
	long-chain fatty-acyl-CoA catabolic process	GO:0036116	ISO
	medium-chain fatty acid biosynthetic process	GO:0051792	IEA
	medium-chain fatty acid biosynthetic process	GO:0051792	ISO
	palmitic acid biosynthetic process	GO:1900535	IEA
	palmitic acid biosynthetic process	GO:1900535	ISO
Subcellular Localization
	nucleoplasm	GO:0005654	IEA
	nucleoplasm	GO:0005654	ISO
	cytoplasm	GO:0005737	IBA
	cytoplasm	GO:0005737	ISO
	mitochondrial matrix	GO:0005759	ISO
	cytosol	GO:0005829	IBA
	cytosol	GO:0005829	IEA
	cytosol	GO:0005829	ISO
	neuron projection	GO:0043005	ISO
	neuronal cell body	GO:0043025	ISO

Experiment description of studies that identified Acot7 in sEVs

Experiment ID

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|HSP90\|HSC70\|MHCI	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

"Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D7 Rep 1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.13-1.25 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting