Gene description for RNU4-2
Gene name RNA, U4 small nuclear 2
Gene symbol RNU4-2
Other names/aliases RNU4-1B
RNU4B1
RNU4C
U4A
U4b
U4c
Species Homo sapiens
 Database cross references - RNU4-2
ExoCarta ExoCarta_26834
Vesiclepedia VP_26834
Entrez Gene 26834
HGNC 10193
 RNU4-2 identified in sEVs derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for RNU4-2
Molecular Function
    U6 snRNA binding GO:0017070 IEA
Biological Process
    spliceosomal tri-snRNP complex assembly GO:0000244 IEA
    formation of quadruple SL/U4/U5/U6 snRNP GO:0000353 IEA
Subcellular Localization
    U4 snRNP GO:0005687 IEA
    U4/U6 x U5 tri-snRNP complex GO:0046540 IEA
 Experiment description of studies that identified RNU4-2 in sEVs
1
Experiment ID 226
MISEV standards
EM
Biophysical techniques
GAPDH|CD9|FLOT1
Enriched markers
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
Particle analysis
Identified molecule snRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for RNU4-2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which RNU4-2 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here