Gene description for Eif2s3x
Gene name eukaryotic translation initiation factor 2, subunit 3, structural gene X-linked
Gene symbol Eif2s3x
Other names/aliases AA409828
AA547477
AI314668
Eif-2gx
Species Mus musculus
 Database cross references - Eif2s3x
ExoCarta ExoCarta_26905
Vesiclepedia VP_26905
Entrez Gene 26905
UniProt Q9Z0N1  
 Eif2s3x identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Eif2s3x
Molecular Function
    tRNA binding GO:0000049 IBA
    translation initiation factor activity GO:0003743 IBA
    translation initiation factor activity GO:0003743 ISO
    translation initiation factor activity GO:0003743 ISS
    GTP binding GO:0005525 IEA
    translation factor activity, RNA binding GO:0008135 ISO
    translation factor activity, RNA binding GO:0008135 ISS
    membrane scission GTPase motor activity GO:1990606 IEA
    methionyl-initiator methionine tRNA binding GO:1990856 ISS
Biological Process
    formation of translation preinitiation complex GO:0001731 IBA
    cytoplasmic translational initiation GO:0002183 ISS
    translational initiation GO:0006413 ISS
Subcellular Localization
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IEA
    eukaryotic translation initiation factor 2 complex GO:0005850 IBA
    eukaryotic translation initiation factor 2 complex GO:0005850 ISO
    eukaryotic translation initiation factor 2 complex GO:0005850 ISS
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
 Experiment description of studies that identified Eif2s3x in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Eif2s3x
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Eed  
Affinity Capture-MS Mus musculus
2 Zbtb7b  
Affinity Capture-MS Mus musculus
3 Gab2  
Affinity Capture-MS Mus musculus
4 Eif2a  
Co-fractionation Mus musculus
5 Kctd13  
Affinity Capture-MS Mus musculus
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