Gene description for Epb4.1
Gene name erythrocyte protein band 4.1
Gene symbol Epb4.1
Other names/aliases 4.1R
AI415518
D4Ertd442e
Elp-1
Elp1
Epb41
mKIAA4056
Species Mus musculus
 Database cross references - Epb4.1
ExoCarta ExoCarta_269587
Vesiclepedia VP_269587
Entrez Gene 269587
UniProt P48193  
 Epb4.1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Epb4.1
Molecular Function
    actin binding GO:0003779 ISO
    actin binding GO:0003779 ISS
    structural molecule activity GO:0005198 IEA
    structural constituent of cytoskeleton GO:0005200 ISO
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IEA
    1-phosphatidylinositol binding GO:0005545 ISO
    spectrin binding GO:0030507 ISO
    spectrin binding GO:0030507 ISS
    phosphoprotein binding GO:0051219 ISO
Biological Process
    regulation of cell shape GO:0008360 ISS
    actin cytoskeleton organization GO:0030036 ISS
    cortical actin cytoskeleton organization GO:0030866 IEA
    actomyosin structure organization GO:0031032 IBA
    positive regulation of protein binding GO:0032092 IGI
    positive regulation of protein binding GO:0032092 ISO
    cell division GO:0051301 IEA
    regulation of calcium ion transport GO:0051924 IMP
    protein-containing complex assembly GO:0065003 ISO
    regulation of intestinal absorption GO:1904478 IMP
    positive regulation of protein localization to cell cortex GO:1904778 ISO
    positive regulation of protein localization to cell cortex GO:1904778 ISS
Subcellular Localization
    nucleus GO:0005634 IEA
    cytosol GO:0005829 ISO
    cytoskeleton GO:0005856 IBA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    cell cortex GO:0005938 ISO
    cell cortex GO:0005938 ISS
    cytoplasmic side of plasma membrane GO:0009898 ISO
    actin cytoskeleton GO:0015629 ISS
    membrane GO:0016020 IDA
    basolateral plasma membrane GO:0016323 IDA
    nuclear body GO:0016604 ISO
    cell junction GO:0030054 ISO
    cortical cytoskeleton GO:0030863 IDA
    cortical cytoskeleton GO:0030863 ISO
    protein-containing complex GO:0032991 ISO
    intercellular bridge GO:0045171 ISO
    mitotic spindle GO:0072686 ISO
 Experiment description of studies that identified Epb4.1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Epb4.1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tmem173  
Proximity Label-MS Mus musculus
2 Sstr3  
Proximity Label-MS Mus musculus
View the network image/svg+xml
 Pathways in which Epb4.1 is involved
PathwayEvidenceSource
Neurexins and neuroligins IEA Reactome
Neuronal System IEA Reactome
Protein-protein interactions at synapses IEA Reactome





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