ExoCarta: Gene summary

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Gene description for Eif2ak1
Gene name	eukaryotic translation initiation factor 2 alpha kinase 1
Gene symbol	Eif2ak1
Other names/aliases	Hri
Species	Rattus norvegicus
Database cross references - Eif2ak1
ExoCarta	ExoCarta_27137
Entrez Gene	27137
UniProt	Q63185
Eif2ak1 identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Eif2ak1

Molecular Function
	protein kinase activity	GO:0004672	ISO
	eukaryotic translation initiation factor 2alpha kinase activity	GO:0004694	ISS
	heme binding	GO:0020037	ISS
	protein homodimerization activity	GO:0042803	ISS
	ATP binding	GO:0005524	IEA
Biological Process
	negative regulation of hemoglobin biosynthetic process	GO:0046986	ISS
	regulation of translational initiation by eIF2 alpha phosphorylation	GO:0010998	ISS
	protein phosphorylation	GO:0006468	TAS
	acute inflammatory response	GO:0002526	ISO
	macrophage differentiation	GO:0030225	ISO
	protoporphyrinogen IX metabolic process	GO:0046501	ISO
	negative regulation of cell proliferation	GO:0008285	ISS
	iron ion homeostasis	GO:0055072	ISO
	translational initiation	GO:0006413	TAS
	regulation of eIF2 alpha phosphorylation by heme	GO:0010999	ISO
	regulation of translation	GO:0006417	ISO
	protein autophosphorylation	GO:0046777	ISS
	regulation of hemoglobin biosynthetic process	GO:0046984	ISO
	phagocytosis	GO:0006909	ISO
	negative regulation of translational initiation	GO:0045947	TAS
Subcellular Localization
	cytoplasm	GO:0005737	ISS

Experiment description of studies that identified Eif2ak1 in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ TSG101\|HSP90\|HSC70	EV Cytosolic markers
✔ MHCI	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D7 Rep 1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.13-1.25 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting