Gene description for GNAT1
Gene name guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 1
Gene symbol GNAT1
Other names/aliases CSNBAD3
GBT1
GNATR
Species Homo sapiens
 Database cross references - GNAT1
ExoCarta ExoCarta_2779
Vesiclepedia VP_2779
Entrez Gene 2779
HGNC 4393
MIM 139330
UniProt P11488  
 GNAT1 identified in sEVs derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for GNAT1
Molecular Function
    acyl binding GO:0000035 ISS
    G protein-coupled receptor binding GO:0001664 IBA
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 NAS
    GTPase activity GO:0003924 TAS
    GTP binding GO:0005525 ISS
    GDP binding GO:0019003 ISS
    protein kinase binding GO:0019901 ISS
    G-protein beta/gamma-subunit complex binding GO:0031683 IBA
    metal ion binding GO:0046872 IEA
Biological Process
    detection of chemical stimulus involved in sensory perception of bitter taste GO:0001580 IEA
    signal transduction GO:0007165 NAS
    adenylate cyclase-modulating G protein-coupled receptor signaling pathway GO:0007188 IBA
    visual perception GO:0007601 TAS
    phototransduction, visible light GO:0007603 IBA
    phototransduction, visible light GO:0007603 IMP
    visual behavior GO:0007632 IEA
    cell population proliferation GO:0008283 IEA
    response to light stimulus GO:0009416 ISS
    dopamine secretion GO:0014046 IEA
    response to organic cyclic compound GO:0014070 IEA
    G protein-coupled opsin signaling pathway GO:0016056 IEA
    regulation of opsin-mediated signaling pathway GO:0022400 TAS
    eye photoreceptor cell development GO:0042462 IEA
    retinal cone cell differentiation GO:0042670 IEA
    detection of light stimulus involved in visual perception GO:0050908 IBA
    detection of light stimulus involved in visual perception GO:0050908 IMP
    sensory perception of umami taste GO:0050917 IEA
    negative regulation of cyclic-nucleotide phosphodiesterase activity GO:0051344 ISS
    retinal rod cell differentiation GO:0060221 IEA
    cellular response to electrical stimulus GO:0071257 IEA
    neural tissue regeneration GO:0097719 IEA
    background adaptation GO:0120302 IEA
Subcellular Localization
    photoreceptor outer segment GO:0001750 IDA
    photoreceptor inner segment GO:0001917 IDA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    heterotrimeric G-protein complex GO:0005834 IBA
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 ISS
    membrane GO:0016020 NAS
    apical plasma membrane GO:0016324 IEA
    photoreceptor connecting cilium GO:0032391 IEA
    photoreceptor outer segment membrane GO:0042622 ISS
    neuronal cell body GO:0043025 IEA
    photoreceptor disc membrane GO:0097381 TAS
 Experiment description of studies that identified GNAT1 in sEVs
1
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GNAT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DNAJC6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 Haus1  
Affinity Capture-MS Mus musculus
3 RPL4 6124
Cross-Linking-MS (XL-MS) Homo sapiens
4 ARL5B  
Affinity Capture-MS Homo sapiens
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