Gene description for ARFGEF1
Gene name ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited)
Gene symbol ARFGEF1
Other names/aliases BIG1
Species Bos taurus
 Database cross references - ARFGEF1
ExoCarta ExoCarta_281022
Entrez Gene 281022
UniProt O46382  
 ARFGEF1 identified in exosomes derived from the following tissue/cell type
Milk 23459212    
 Gene ontology annotations for ARFGEF1
Molecular Function
    ARF guanyl-nucleotide exchange factor activity GO:0005086 IEA
    protein kinase A regulatory subunit binding GO:0034237 ISS
Biological Process
    positive regulation of protein glycosylation in Golgi GO:0090284 ISS
    regulation of establishment of cell polarity GO:2000114 ISS
    negative regulation of GTPase activity GO:0034260 ISS
    endomembrane system organization GO:0010256 ISS
    protein transport GO:0015031 IEA
    Golgi organization GO:0007030 ISS
    positive regulation of wound healing GO:0090303 ISS
    negative regulation of actin filament polymerization GO:0030837 ISS
    positive regulation of GTPase activity GO:0043547 IEA
Subcellular Localization
    nucleolus GO:0005730 ISS
    perinuclear region of cytoplasm GO:0048471 IEA
    cytosol GO:0005829 ISS
    Golgi membrane GO:0000139 ISS
    trans-Golgi network GO:0005802 ISS
    small nuclear ribonucleoprotein complex GO:0030532 ISS
    nuclear matrix GO:0016363 IEA
 Experiment description of studies that identified ARFGEF1 in exosomes
1
Experiment ID 213
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23459212    
Organism Bos taurus
Experiment description Bovine milk proteome: Quantitative changes in normal milk exosomes, milk fat globule membranes and whey proteomes resulting from Staphylococcus aureus mastitis.
Authors Reinhardt TA, Sacco RE, Nonnecke BJ, Lippolis JD.
Journal name J Proteomics
Publication year 2013
Sample Milk
Sample name Staphylococcus aureus-infected-Milk
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Filtration
Sucrose density gradient
FACS
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ARFGEF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which ARFGEF1 is involved
No pathways found





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