Gene description for GAPDH
Gene name glyceraldehyde-3-phosphate dehydrogenase
Gene symbol GAPDH
Other names/aliases GAPD
Species Bos taurus
 Database cross references - GAPDH
ExoCarta ExoCarta_281181
Entrez Gene 281181
UniProt P10096  
 GAPDH identified in exosomes derived from the following tissue/cell type
Milk 23459212    
Serum 19327352    
 Gene ontology annotations for GAPDH
Molecular Function
    glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity GO:0004365 ISS
    microtubule binding GO:0008017 ISS
    peptidyl-cysteine S-nitrosylase activity GO:0035605 ISS
    protein binding GO:0005515 IPI
    NADP binding GO:0050661 IEA
    NAD binding GO:0051287 IEA
Biological Process
    neuron apoptotic process GO:0051402 ISS
    microtubule cytoskeleton organization GO:0000226 ISS
    oxidation-reduction process GO:0055114 IEA
    protein stabilization GO:0050821 ISS
    glycolytic process GO:0006096 IEA
    glucose metabolic process GO:0006006 IEA
    peptidyl-cysteine S-trans-nitrosylation GO:0035606 ISS
    regulation of translation GO:0006417 IEA
Subcellular Localization
    microtubule cytoskeleton GO:0015630 ISS
    GAIT complex GO:0097452 ISS
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 ISS
    nucleus GO:0005634 ISS
 Experiment description of studies that identified GAPDH in exosomes
1
Experiment ID 213
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23459212    
Organism Bos taurus
Experiment description Bovine milk proteome: Quantitative changes in normal milk exosomes, milk fat globule membranes and whey proteomes resulting from Staphylococcus aureus mastitis.
Authors Reinhardt TA, Sacco RE, Nonnecke BJ, Lippolis JD.
Journal name J Proteomics
Publication year 2013
Sample Milk
Sample name Staphylococcus aureus-infected-Milk
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Filtration
Sucrose density gradient
FACS
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 187
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19327352    
Organism Bos taurus
Experiment description Anchorage-independent growth of breast carcinoma cells is mediated by serum exosomes.
Authors Ochieng J, Pratap S, Khatua AK, Sakwe AM.
Journal name Exp Cell Res
Publication year 2009
Sample Serum
Sample name Normal - Fetal bovine serum
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GAPDH
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which GAPDH is involved
PathwayEvidenceSource
Gluconeogenesis IEA Reactome
Glycolysis IEA Reactome





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