Gene description for GAPDH
Gene name glyceraldehyde-3-phosphate dehydrogenase
Gene symbol GAPDH
Other names/aliases GAPD
Species Bos taurus
 Database cross references - GAPDH
ExoCarta ExoCarta_281181
Vesiclepedia VP_281181
Entrez Gene 281181
UniProt P10096  
 GAPDH identified in sEVs derived from the following tissue/cell type
Milk 23459212    
Serum 19327352    
 Gene ontology annotations for GAPDH
Molecular Function
    oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor GO:0016620 IEA
    NADP binding GO:0050661 IEA
    NAD binding GO:0051287 IEA
Biological Process
    glucose metabolic process GO:0006006 IEA
 Experiment description of studies that identified GAPDH in sEVs
1
Experiment ID 213
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23459212    
Organism Bos taurus
Experiment description "Bovine milk proteome: Quantitative changes in normal milk exosomes, milk fat globule membranes and whey proteomes resulting from Staphylococcus aureus mastitis."
Authors "Reinhardt TA, Sacco RE, Nonnecke BJ, Lippolis JD."
Journal name J Proteomics
Publication year 2013
Sample Milk
Sample name Staphylococcus aureus-infected-Milk
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Filtration
Sucrose density gradient
FACS
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 187
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19327352    
Organism Bos taurus
Experiment description Anchorage-independent growth of breast carcinoma cells is mediated by serum exosomes.
Authors "Ochieng J, Pratap S, Khatua AK, Sakwe AM."
Journal name Exp Cell Res
Publication year 2009
Sample Serum
Sample name Normal - Fetal bovine serum
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GAPDH
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TPPP  
Affinity Capture-MS Bos taurus
Affinity Capture-Western Bos taurus
Reconstituted Complex Bos taurus
View the network image/svg+xml
 Pathways in which GAPDH is involved
PathwayEvidenceSource
Gluconeogenesis IEA Reactome
Glucose metabolism IEA Reactome
Glycolysis IEA Reactome
Metabolism IEA Reactome
Metabolism of carbohydrates IEA Reactome





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