Gene description for RAC1
Gene name ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
Gene symbol RAC1
Other names/aliases -
Species Bos taurus
 Database cross references - RAC1
ExoCarta ExoCarta_281440
Entrez Gene 281440
UniProt P62998  
 RAC1 identified in exosomes derived from the following tissue/cell type
Milk 23459212    
 Gene ontology annotations for RAC1
Molecular Function
    GTP binding GO:0005525 ISS
    GTPase activity GO:0003924 IEA
    Rho GDP-dissociation inhibitor binding GO:0051022 ISS
    thioesterase binding GO:0031996 IEA
    protein binding GO:0005515 IPI
    GTP-dependent protein binding GO:0030742 IEA
Biological Process
    regulation of cell migration GO:0030334 ISS
    Wnt signaling pathway, planar cell polarity pathway GO:0060071 IEA
    negative regulation of interleukin-23 production GO:0032707 IEA
    embryonic olfactory bulb interneuron precursor migration GO:0021831 IEA
    positive regulation of focal adhesion assembly GO:0051894 IEA
    epithelial cell morphogenesis GO:0003382 IEA
    positive regulation of stress fiber assembly GO:0051496 IEA
    positive regulation of protein phosphorylation GO:0001934 ISS
    cochlea morphogenesis GO:0090103 IEA
    semaphorin-plexin signaling pathway GO:0071526 ISS
    positive regulation of actin filament polymerization GO:0030838 IEA
    lamellipodium assembly GO:0030032 IEA
    positive regulation of neutrophil chemotaxis GO:0090023 IEA
    auditory receptor cell morphogenesis GO:0002093 IEA
    small GTPase mediated signal transduction GO:0007264 IEA
    positive regulation of lamellipodium assembly GO:0010592 IEA
    substrate adhesion-dependent cell spreading GO:0034446 IEA
    hyperosmotic response GO:0006972 IEA
    axon guidance GO:0007411 IEA
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 IEA
    cerebral cortex radially oriented cell migration GO:0021799 IEA
    dopaminergic neuron differentiation GO:0071542 IEA
    localization within membrane GO:0051668 IEA
    ruffle assembly GO:0097178 IEA
    dendrite morphogenesis GO:0048813 IEA
    G-protein coupled receptor signaling pathway GO:0007186 IEA
    protein localization to plasma membrane GO:0072659 IEA
    positive regulation of phosphatidylinositol 3-kinase activity GO:0043552 IEA
    anatomical structure arrangement GO:0048532 IEA
    regulation of respiratory burst GO:0060263 IEA
    metabolic process GO:0008152 IEA
    engulfment of apoptotic cell GO:0043652 IEA
    actin filament polymerization GO:0030041 IEA
    cell-cell junction organization GO:0045216 IEA
    cell motility GO:0048870 ISS
Subcellular Localization
    cytosol GO:0005829 ISS
    cytoplasmic ribonucleoprotein granule GO:0036464 IEA
    phagocytic cup GO:0001891 IEA
    trans-Golgi network GO:0005802 IEA
    extracellular exosome GO:0070062 IEA
    extrinsic component of plasma membrane GO:0019897 IEA
    lamellipodium GO:0030027 IEA
    ruffle membrane GO:0032587 IEA
    focal adhesion GO:0005925 IEA
    melanosome GO:0042470 IEA
    actin filament GO:0005884 IEA
    membrane GO:0016020 ISS
 Experiment description of studies that identified RAC1 in exosomes
1
Experiment ID 213
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23459212    
Organism Bos taurus
Experiment description Bovine milk proteome: Quantitative changes in normal milk exosomes, milk fat globule membranes and whey proteomes resulting from Staphylococcus aureus mastitis.
Authors Reinhardt TA, Sacco RE, Nonnecke BJ, Lippolis JD.
Journal name J Proteomics
Publication year 2013
Sample Milk
Sample name Staphylococcus aureus-infected-Milk
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Filtration
Sucrose density gradient
FACS
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RAC1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which RAC1 is involved
PathwayEvidenceSource
Activation of Rac IEA Reactome
CD28 dependent Vav1 pathway IEA Reactome
DAP12 signaling IEA Reactome
DCC mediated attractive signaling IEA Reactome
DSCAM interactions IEA Reactome
EPH-ephrin mediated repulsion of cells IEA Reactome
EPHB-mediated forward signaling IEA Reactome
Ephrin signaling IEA Reactome
Factors involved in megakaryocyte development and platelet production IEA Reactome
FCERI mediated MAPK activation IEA Reactome
G alpha (12/13) signalling events IEA Reactome
GPVI-mediated activation cascade IEA Reactome
Inactivation of Cdc42 and Rac IEA Reactome
NRAGE signals death through JNK IEA Reactome
PCP/CE pathway IEA Reactome
Regulation of actin dynamics for phagocytic cup formation IEA Reactome
Rho GTPase cycle IEA Reactome
RHO GTPases Activate Formins IEA Reactome
RHO GTPases activate IQGAPs IEA Reactome
RHO GTPases activate KTN1 IEA Reactome
RHO GTPases Activate NADPH Oxidases IEA Reactome
RHO GTPases activate PAKs IEA Reactome
RHO GTPases Activate WASPs and WAVEs IEA Reactome
Sema3A PAK dependent Axon repulsion IEA Reactome
SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion IEA Reactome
Sema4D induced cell migration and growth-cone collapse IEA Reactome
Sema4D mediated inhibition of cell attachment and migration IEA Reactome
Signal transduction by L1 IEA Reactome
Signaling by SCF-KIT IEA Reactome
Translocation of GLUT4 to the plasma membrane IEA Reactome
VEGFA-VEGFR2 Pathway IEA Reactome
VEGFR2 mediated vascular permeability IEA Reactome





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