Gene description for HSPA8
Gene name heat shock 70kDa protein 8
Gene symbol HSPA8
Other names/aliases HSPA10
Hsc70
Species Bos taurus
 Database cross references - HSPA8
ExoCarta ExoCarta_281831
Entrez Gene 281831
UniProt P19120  
 HSPA8 identified in exosomes derived from the following tissue/cell type
Milk 23459212    
 Gene ontology annotations for HSPA8
Molecular Function
    ATPase activity, coupled GO:0042623 IEA
    unfolded protein binding GO:0051082 IEA
    MHC class II protein complex binding GO:0023026 IEA
    protein binding GO:0005515 IPI
    heat shock protein binding GO:0031072 IEA
    ATP binding GO:0005524 IEA
    poly(A) RNA binding GO:0044822 IEA
    ubiquitin protein ligase binding GO:0031625 IEA
    G-protein coupled receptor binding GO:0001664 IEA
Biological Process
    mRNA processing GO:0006397 IEA
    RNA splicing GO:0008380 IEA
    chaperone mediated protein folding requiring cofactor GO:0051085 IEA
    clathrin coat disassembly GO:0072318 IEA
    negative regulation of transcription, DNA-templated GO:0045892 ISS
    protein refolding GO:0042026 IEA
    regulation of cell cycle GO:0051726 IEA
    transcription, DNA-templated GO:0006351 IEA
    positive regulation of mRNA splicing, via spliceosome GO:0048026 IEA
    negative regulation of fibril organization GO:1902904 IEA
Subcellular Localization
    melanosome GO:0042470 IEA
    focal adhesion GO:0005925 IEA
    cytosol GO:0005829 IEA
    nucleolus GO:0005730 IEA
    Prp19 complex GO:0000974 ISS
    ribonucleoprotein complex GO:0030529 ISS
    blood microparticle GO:0072562 IEA
    ubiquitin ligase complex GO:0000151 IEA
    plasma membrane GO:0005886 IEA
    extracellular exosome GO:0070062 IEA
    nucleus GO:0005634 ISS
    spliceosomal complex GO:0005681 IEA
 Experiment description of studies that identified HSPA8 in exosomes
1
Experiment ID 213
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23459212    
Organism Bos taurus
Experiment description Bovine milk proteome: Quantitative changes in normal milk exosomes, milk fat globule membranes and whey proteomes resulting from Staphylococcus aureus mastitis.
Authors Reinhardt TA, Sacco RE, Nonnecke BJ, Lippolis JD.
Journal name J Proteomics
Publication year 2013
Sample Milk
Sample name Staphylococcus aureus-infected-Milk
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Filtration
Sucrose density gradient
FACS
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HSPA8
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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