Gene description for Rffl
Gene name ring finger and FYVE-like domain containing E3 ubiquitin protein ligase
Gene symbol Rffl
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Rffl
ExoCarta ExoCarta_282844
Entrez Gene 282844
UniProt Q8CIN9  
 Rffl identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Rffl
Molecular Function
    protease binding GO:0002020 ISO
    ubiquitin protein ligase activity GO:0061630 ISS
    zinc ion binding GO:0008270 IEA
    molecular_function GO:0003674 ND
    protein kinase binding GO:0019901 ISO
    ligase activity GO:0016874 IEA
    p53 binding GO:0002039 ISO
    ubiquitin protein ligase binding GO:0031625 ISO
Biological Process
    negative regulation of signal transduction by p53 class mediator GO:1901797 ISS
    negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis GO:2001271 ISS
    intracellular protein transport GO:0006886 ISO
    negative regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902042 ISS
    protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:0042787 ISS
    protein K48-linked ubiquitination GO:0070936 ISS
    negative regulation of tumor necrosis factor-mediated signaling pathway GO:0010804 ISS
    regulation of fibroblast migration GO:0010762 ISS
    ubiquitin-dependent protein catabolic process GO:0006511 ISS
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 ISS
    biological_process GO:0008150 ND
Subcellular Localization
    cellular_component GO:0005575 ND
    endosome membrane GO:0010008 ISS
    recycling endosome membrane GO:0055038 IEA
    cytoplasm GO:0005737 ISS
    lysosome GO:0005764 ISO
    cytosol GO:0005829 IEA
    membrane GO:0016020 ISO
    cytoplasmic membrane-bounded vesicle GO:0016023 ISO
    plasma membrane GO:0005886 ISS
    tumor necrosis factor receptor superfamily complex GO:0002947 ISS
    Golgi membrane GO:0000139 ISO
 Experiment description of studies that identified Rffl in exosomes
1
Experiment ID 90
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSC70|HSP90|TSG101
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Rffl
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Rffl is involved
No pathways found





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