Gene description for Gnat3
Gene name guanine nucleotide binding protein, alpha transducing 3
Gene symbol Gnat3
Other names/aliases gnat-3
Species Rattus norvegicus
 Database cross references - Gnat3
ExoCarta ExoCarta_286924
Entrez Gene 286924
UniProt P29348  
 Gnat3 identified in exosomes derived from the following tissue/cell type
Cortical neurones 16446100    
 Gene ontology annotations for Gnat3
Molecular Function
    G-protein beta/gamma-subunit complex binding GO:0031683 IBA
    signal transducer activity GO:0004871 IBA
    metal ion binding GO:0046872 IEA
    GTPase activity GO:0003924 ISO
    GTP binding GO:0005525 IDA
    G-protein coupled receptor binding GO:0001664 IBA
Biological Process
    sensory perception of bitter taste GO:0050913 ISO
    metabolic process GO:0008152 IEA
    adenylate cyclase-modulating G-protein coupled receptor signaling pathway GO:0007188 IBA
    sensory perception of umami taste GO:0050917 ISO
    sensory perception of sweet taste GO:0050916 ISO
    detection of chemical stimulus involved in sensory perception of bitter taste GO:0001580 IBA
    visual perception GO:0007601 IEA
    response to nicotine GO:0035094 IEP
    G-protein coupled receptor signaling pathway GO:0007186 ISO
    sensory perception of taste GO:0050909 ISO
Subcellular Localization
    photoreceptor outer segment GO:0001750 IBA
    photoreceptor inner segment GO:0001917 IBA
    cytoplasm GO:0005737 ISO
    protein complex GO:0043234 ISO
    heterotrimeric G-protein complex GO:0005834 ISO
    apical plasma membrane GO:0016324 IDA
    axoneme GO:0005930 IDA
    acrosomal vesicle GO:0001669 IDA
 Experiment description of studies that identified Gnat3 in exosomes
1
Experiment ID 30
ISEV standards
EM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
FLOT
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16446100    
Organism Rattus norvegicus
Experiment description Exosomes are released by cultured cortical neurones.
Authors Faure J, Lachenal G, Court M, Hirrlinger J, Chatellard-Causse C, Blot B, Grange J, Schoehn G, Goldberg Y, Boyer V, Kirchhoff F, Raposo G, Garin J, Sadoul R
Journal name MCN
Publication year 2006
Sample Cortical neurones
Sample name Cortical neurones
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Gnat3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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