Gene description for Gnat3
Gene name guanine nucleotide binding protein, alpha transducing 3
Gene symbol Gnat3
Other names/aliases gnat-3
Species Rattus norvegicus
 Database cross references - Gnat3
ExoCarta ExoCarta_286924
Vesiclepedia VP_286924
Entrez Gene 286924
UniProt P29348  
 Gnat3 identified in sEVs derived from the following tissue/cell type
Cortical neurones 16446100    
 Gene ontology annotations for Gnat3
Molecular Function
    G protein-coupled receptor binding GO:0001664 IBA
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IDA
    GTPase activity GO:0003924 ISO
    GTP binding GO:0005525 IDA
    G-protein beta/gamma-subunit complex binding GO:0031683 IBA
    metal ion binding GO:0046872 IEA
Biological Process
    G protein-coupled receptor signaling pathway GO:0007186 ISO
    adenylate cyclase-modulating G protein-coupled receptor signaling pathway GO:0007188 IBA
    visual perception GO:0007601 IEA
    phototransduction, visible light GO:0007603 IBA
    response to nicotine GO:0035094 IEP
    detection of light stimulus involved in visual perception GO:0050908 IBA
    sensory perception of taste GO:0050909 ISO
    sensory perception of bitter taste GO:0050913 ISO
    sensory perception of sweet taste GO:0050916 IBA
    sensory perception of sweet taste GO:0050916 ISO
    sensory perception of umami taste GO:0050917 ISO
Subcellular Localization
    acrosomal vesicle GO:0001669 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    heterotrimeric G-protein complex GO:0005834 IBA
    heterotrimeric G-protein complex GO:0005834 ISO
    plasma membrane GO:0005886 TAS
    axoneme GO:0005930 IDA
    apical plasma membrane GO:0016324 IDA
    protein-containing complex GO:0032991 ISO
 Experiment description of studies that identified Gnat3 in sEVs
1
Experiment ID 30
MISEV standards
EM
Biophysical techniques
Alix|TSG101|FLOT
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16446100    
Organism Rattus norvegicus
Experiment description Exosomes are released by cultured cortical neurones.
Authors "Faure J, Lachenal G, Court M, Hirrlinger J, Chatellard-Causse C, Blot B, Grange J, Schoehn G, Goldberg Y, Boyer V, Kirchhoff F, Raposo G, Garin J, Sadoul R"
Journal name MCN
Publication year 2006
Sample Cortical neurones
Sample name Cortical neurones
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Gnat3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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