Experiment ID |
34 |
ISEV standards |
✔
EM|IEM
|
EV Biophysical techniques |
✔
TSG101
|
EV Cytosolic markers |
✔
CD63|MHCI|MHCII
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
19190083
|
Organism |
Homo sapiens |
Experiment description |
Characterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium: a possible role in innate defense. |
Authors |
Kesimer M, Scull M, Brighton B, Demaria G, Burns K, O'Neal W, Pickles RJ, Sheehan JK |
Journal name |
FASEB
|
Publication year |
2009 |
Sample |
Tracheobronchial cells |
Sample name |
Tracheobronchial epithelial cells |
Isolation/purification methods |
Differential centrifugation Filtration Sucrose density gradient |
Flotation density |
1.16 -1.18 g/mL
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
Mass spectrometry [QTOF] Western blotting Immunoelectron Microscopy FACS |